Anti-RHOC antibody [2E12]
| Name | Anti-RHOC antibody [2E12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab54837 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 2E12 |
| Applications | FC WB |
| Species Reactivities | Rat, Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-194 of Human RHOC |
| Description | Mouse Monoclonal |
| Gene | RHOC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
RHOC antibody (ab54837) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
![Overlay histogram showing HCT116 cells stained with ab54837 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54837, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/22897_RHOC-Primary-antibodies-ab54837-3.jpg)
Overlay histogram showing HCT116 cells stained with ab54837 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54837, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Use of synthetic isoprenoids to target protein prenylation and Rho GTPases in - Use of synthetic isoprenoids to target protein prenylation and Rho GTPases in
Chen M, Knifley T, Subramanian T, Spielmann HP, O'Connor KL. PLoS One. 2014 Feb 26;9(2):e89892.
MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for - MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for
Passadouro M, Pedroso de Lima MC, Faneca H. Int J Nanomedicine. 2014 Jul 3;9:3203-17.
DLC1 induces expression of E-cadherin in prostate cancer cells through Rho - DLC1 induces expression of E-cadherin in prostate cancer cells through Rho
Tripathi V, Popescu NC, Zimonjic DB. Oncogene. 2014 Feb 6;33(6):724-33.
The activation of RhoC in vascular endothelial cells is required for the S1P - The activation of RhoC in vascular endothelial cells is required for the S1P
Del Galdo S, Vettel C, Heringdorf DM, Wieland T. Cell Signal. 2013 Dec;25(12):2478-84.
Mesenchymal stem cells promote mammosphere formation and decrease E-cadherin in - Mesenchymal stem cells promote mammosphere formation and decrease E-cadherin in
Klopp AH, Lacerda L, Gupta A, Debeb BG, Solley T, Li L, Spaeth E, Xu W, Zhang X, Lewis MT, Reuben JM, Krishnamurthy S, Ferrari M, Gaspar R, Buchholz TA, Cristofanilli M, Marini F, Andreeff M, Woodward WA. PLoS One. 2010 Aug 16;5(8):e12180.
Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus - Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus
Quinn K, Brindley MA, Weller ML, Kaludov N, Kondratowicz A, Hunt CL, Sinn PL, McCray PB Jr, Stein CS, Davidson BL, Flick R, Mandell R, Staplin W, Maury W, Chiorini JA. J Virol. 2009 Oct;83(19):10176-86.
NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell - NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell
Murray D, Horgan G, Macmathuna P, Doran P. Br J Cancer. 2008 Oct 21;99(8):1322-9.