Anti-RhoGAP antibody [EP489Y]

Name	Anti-RhoGAP antibody [EP489Y]
Supplier	Abcam
Catalog	ab32328
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EP489Y
Applications	WB IHC-P FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (Human) from RhoGAP
Description	Rabbit Monoclonal
Gene	ARHGAP5
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Overlay histogram showing A431 cells stained with ab32328 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32328, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilution + Mouse MEFs whole cell lysate at 50 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilutionLane 1 : Hela cell lysateLane 2 : Jurkat cell lysateLane 3 : MCF-7 cell lysateLane 4 : K562 cell lysateLane 5 : A431 cell lysateLane 6 : 293 cell lysateLane 7 : NIH 3T3 cell lysateLane 8 : PC12 cell lysate

ab32328, at a 1/100 dilution, staining human RhoGAP in stomach carcinoma by Immunohistochemistry, Paraffin embedded tissueab32328, at a 1/100 dilution, staining human RhoGAP in stomach carcinoma by Immunohistochemistry, Paraffin embedded tissue

Product References

p140Cap regulates memory and synaptic plasticity through Src-mediated and - p140Cap regulates memory and synaptic plasticity through Src-mediated and

Repetto D, Camera P, Melani R, Morello N, Russo I, Calcagno E, Tomasoni R, Bianchi F, Berto G, Giustetto M, Berardi N, Pizzorusso T, Matteoli M, Di Stefano P, Missler M, Turco E, Di Cunto F, Defilippi P. J Neurosci. 2014 Jan 22;34(4):1542-53.