ARHGAP5

Gene Symbol	ARHGAP5
Entrez Gene	394
Alt Symbol	GFI2, RhoGAP5, p190-B, p190BRhoGAP
Species	Human
Gene Type	protein-coding
Description	Rho GTPase activating protein 5
Other Description	growth factor independent 2\|p100 RasGAP-associated p105 protein\|p105 RhoGAP\|rho GTPase-activating protein 5\|rho-type GTPase-activating protein 5
Swissprots	D3DS89 Q05BU8 D3DS90 A1L376 Q59ER0 Q6DHZ3 A1L375 Q13017 A8KAA1 Q05BE8
Accessions	CCQ43130 EAW65935 EAW65936 EAW65937 EAW65938 Q13017 AB209751 BAD92988 AK023014 AK023468 AK292966 BAF85655 AK307454 AL045112 AY316523 AAP83192 BC032723 AAH32723 BC050059 AAH50059 BC075799 AAH75799 BC129928 AAI29929 BC129929 AAI29930 CA945100 U17032 AAA95963 XM_005267635 XP_005267692 XM_005267636 XP_005267693 NM_001030055 NP_001025226 NM_001173 NP_001164
Function	GTPase-activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of RASA1/p120GAP.
Subcellular Location	Cytoplasm. Membrane; Peripheral membrane protein. Note=Also membrane-associated when found in fibrillar patterns that colocalize with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin.
Tissue Specificity	Expressed in kidney, brain, liver and lung.
Top Pathways	Focal adhesion, Leukocyte transendothelial migration

p190-B RhoGAP Antibody - 2562 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot anlaysis of extracts from MCF-7 (human), C2C12 (mouse), and BAEC (bovine) cells, using p190-B RhoGAP Antibody.

Rho GAP p190-B (G-11) - sc-393241 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Rho GAP p190-B (G-11): sc-393241. Western blot analysis of Rho GAP p190-B expression in Jurkat (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.

Rho GAP p190-B (H-160) - sc-30206 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Rho GAP p190-B (S-19) - sc-23620 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Rho GAP p190-B (S-19): sc-23620. Western blot analysis of Rho GAP p190-B expression in HEK293 whole cell lysate.

Rho GAP p190-B (54) - sc-136187 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Mouse
Clonality	Monoclonal

Anti-Arhgap5 antibody - ab199160 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Arhgap5 with ab199160 at 1/25 dilution.

Anti-Arhgap5 antibody - ab118948 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Arhgap5 antibody (ab118948) at 1 µg/ml + Mouse thymus lysate at 10 µg

Anti-RhoGAP antibody - ab50860 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RhoGAP antibody - ab93375 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-RhoGAP antibody (ab93375) at 1/100 dilutionLane 1 : 293 cell line lysateLane 2 : Mouse cerebellum tissue lysateLysates/proteins at 35 µg per lane.

Anti-RhoGAP antibody [EP489Y] - ab32328 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EP489Y

Overlay histogram showing A431 cells stained with ab32328 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32328, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-ARHGAP5 / RhoGAP5 Antibody (aa180-610) - LS-C38888 from LifeSpan Bioscience

Prices	$475.00
Sizes	200 µg
Host	Mouse
Clonality	Monoclonal

Anti-ARHGAP5 / RhoGAP5 Antibody (Lys296) - LS-C84048 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ARHGAP5 / RhoGAP5 Antibody (N-Terminus) - LS-C145757 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ARHGAP5 antibody LS-C145757 Western blot of Mouse Thymus lysate. Antibody concentration 1 ug/ml.

Anti-ARHGAP5 / RhoGAP5 Antibody - LS-C332668 from LifeSpan Bioscience

Prices	$305.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of HeLa cell line, using ARHGAP5 antibody.

Anti-ARHGAP5 / RhoGAP5 Antibody (Internal, clone EP489Y) - LS-C49599 from LifeSpan Bioscience

Prices	$450.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EP489Y

IHC of paraffin-embedded human stomach carcinoma using anti-p190-B RhoGAP antibody.

Anti-ARHGAP5 / RhoGAP5 Antibody (aa29-210) - LS-C179088 from LifeSpan Bioscience

Prices	$255.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

RhoGAP Antibody - NBP2-20158 from Novus Biologicals

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: RhoGAP Antibody [NBP2-20158] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:2000.

Anti-ARHGAP5 antibody produced in rabbit - SAB2700585 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-ARHGAP5: <B>Cat. No. SAB2700585</B>: Immunohistochemical analysis of paraffin-embedded H441 xenograft, using RhoGAP antibody at 1:500 dilution.

Anti-p190-B RhoGAP (C-terminal region) antibody produced in rabbit - P0045 from Sigma-Aldrich

Prices	$57.50, $332.50
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

$Immunoblotting Lysates of various tissues and cell lines (lanes 1-5) were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-p190-B RhoGAP (C-terminal region) (Cat. No. P0045) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Rat brain (S1 fraction) 2. Mouse brain (S1 fraction) 3. PC-12 4. C2C12 5. Rat brain (S1 fraction). Antibody + p190-B RhoGAP immunizing peptide (human, 1187-1202)$

Anti-ARHGAP5 antibody produced in rabbit - HPA046993 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-ARHGAP5:<B>Cat. No. HPA046993</B>: Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.

RHG05 Antibody - C-terminal region (ARP75928_P050) - ARP75928_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

p190-B-RhoGAP-Antibody - AJ1567a from Abgent, a WuXi AppTec company

Host	Rabbit
Clonality	Monoclonal
Clone	EP489Y

A. Western blot analysis of anti-p190-B RhoGAP RabMAb (Cat. #AJ1567a) 1:500 dilution A-Hela B-Jurkat C-MCF-7 D-K562 E-A431 F-293 G-NIH 3T3 H-PC12

p190-B RhoGAP antibody [EP489Y] - GTX61259 from GeneTex

Host	Rabbit
Clonality	Monoclonal
Clone	EP489Y

Anti-p190-B RhoGAP antibody [EP489Y] used in IHC (Formalin-fixed paraffin-embedded sections). GTX61259

Anti -ARHGAP5 (Rho-type GTPase-activating Protein 5, Rho GTPase-activating Protein 5,GFI2, p190-B, RhoGAP5, p190BRhoGAP) - 209587 from USBiological Life Science

Host	Rabbit
Clonality	Polyclonal

ARHGAP5 antibody - orb78402 from Biorbyt

Prices	$425.00
Sizes	100 μg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of fetal kidney lysate using ARHGAP5 antibody

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