Anti-RhoGDI antibody [2G3]

• Supplier: Abcam
• Catalog: ab135252
• Prices: $392.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 2G3
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Monkey, Rat, Bovine
• Antigen: Purified recombinant full length Human RhoGDI (amino acids 1-204) expressed in E
• Description: Mouse Monoclonal
• Gene: ARHGDIA
• Conjugate: Unconjugated

Product images

Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilution + Human RhoGDI recombinant protein.

All lanes : Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : NIH3T3 cell lysateLane 4 : C6 cell lysateLane 5 : K562 cell lysateLane 6 : COS7 cell lysate

Immunofluorescence analysis of HepG2 cells using ab135252 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric analysis of HeLa cells using ab135252 at 1/200 dilution (green) and negative control (red).

Immunofluorescence analysis of rat lung epithelial cells, staining RhoGDI with ab135252. Cells were fixed with paraformaldehyde, permeabilized with 0.2% saponin and blocked with 10% serum for 10 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 10% serum, 0.2% saponin in PBS) for 1 hour at 23°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/400) was used as the secondary antibody. See Abreview