Anti-RICTOR antibody
| Name | Anti-RICTOR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56578 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: MAAIGRGRSL KNLRVRGRND SGEENVPLDL TREPSDNLRE ILQNVARLQG VSNMRKLGHL NNFTKLLCDI GHSEEKLGFH YEDIIICLRL ALLNEAKE, corresponding to amino acids 1-99 of Human RICTOR Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | RICTOR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-RICTOR antibody (ab56578) at 1 µg/ml + Immunogen recombinant fragment 1-99aaObserved band size : 36.52 kDa (why is the actual band size different from the predicted?)
RICTOR antibody (ab56578) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
RICTOR antibody (ab56578) at 1ug/lane + HeLa cell lysate at 25ug/lane.
See Abreview
![Overlay histogram showing HeLa cells stained with ab56578 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23192_RICTOR-Primary-antibodies-ab56578-6.jpg)
Overlay histogram showing HeLa cells stained with ab56578 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Characteristic mTOR activity in Hodgkin-lymphomas offers a potential therapeutic - Characteristic mTOR activity in Hodgkin-lymphomas offers a potential therapeutic
Mark A, Hajdu M, Varadi Z, Sticz TB, Nagy N, Csomor J, Berczi L, Varga V, Csoka M, Kopper L, Sebestyen A. BMC Cancer. 2013 May 22;13:250.
Role of the integrin-linked kinase (ILK)/Rictor complex in TGFbeta-1-induced - Role of the integrin-linked kinase (ILK)/Rictor complex in TGFbeta-1-induced
Serrano I, McDonald PC, Lock FE, Dedhar S. Oncogene. 2013 Jan 3;32(1):50-60.
TGF-beta-induced activation of mTOR complex 2 drives epithelial-mesenchymal - TGF-beta-induced activation of mTOR complex 2 drives epithelial-mesenchymal
Lamouille S, Connolly E, Smyth JW, Akhurst RJ, Derynck R. J Cell Sci. 2012 Mar 1;125(Pt 5):1259-73.