All lanes : Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilutionLane 1 : HEK293 cell lysate, non-transfectedLane 2 : HEK293 cell lysate, transfected with RING1 (aa 79-263)-hIgGFc
Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilution + Human RING1 recombinant protein
Immunofluorescent analysis of HeLa cells labeling RING1 with ab181756 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescent analysis of A431 cells labeling RING1 with ab181756 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.