All lanes : Anti-RING2 / RING1B / RNF2 antibody (ab106305) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 5 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell LysateLane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 7 : CHO-K1 cell lysate Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-RING2 / RING1B / RNF2 antibody (ab106305) at 1 µg/mlLane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 2 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.