![Overlay histogram showing HEK293 cells stained with ab131638 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131638, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23389_ab131638-9-ab131638FC.jpg)
Overlay histogram showing HEK293 cells stained with ab131638 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131638, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-RIP antibody [2D6] (ab131638) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RIP cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23390_RIP-Primary-antibodies-ab131638-1.jpg)
All lanes : Anti-RIP antibody [2D6] (ab131638) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RIP cDNALysates/proteins at 5 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling RIP using ab131638 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling RIP using ab131638 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RIP using ab131638 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling RIP using ab131638 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labelling RIP using ab131638 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labelling RIP using ab131638 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labelling RIP using ab131638 at 1/150 dilution.