All lanes : Anti-RIP3 antibody (ab62344) at 1 µg/mlLane 1 : Whole cell lysate in the absence of RIP3 peptideLane 2 : Whole cell lysate in the presence of RIP3 peptide
ab62344 at 5 µg/ml staining rat kidney tissue by IHC-P.
Immunofluorescence of RIP3 in Rat Kidney cells using ab62344 at 20 ug/ml.
ICC/IF image of ab62344 stained SV40LT-SMC cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62344, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.