Anti-RIP3 antibody
| Name | Anti-RIP3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56164 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB IHC-P |
| Species Reactivities | Mouse, Human, Rat, Horse, Cat, Dog, Pig |
| Antigen | Synthetic peptide corresponding to Human RIP3 aa 168-217 |
| Blocking Peptide | Human RIP3 peptide |
| Description | Rabbit Polyclonal |
| Gene | RIPK3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![RIP3 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RIP3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56164.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 65kDa; RIP3.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23430_RIP3-Primary-antibodies-ab56164-2.jpg)
RIP3 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RIP3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56164.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 65kDa; RIP3.
All lanes : Anti-RIP3 antibody (ab56164) at 1.25 µg/mlLane 1 : Molecular weight markersLane 2 : Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling RIP3 with ab56164 at 8µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue sections labeling RIP3 with ab56164 at 8µg/ml.
Product References
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Gao X, Zhang H, Zhuang W, Yuan G, Sun T, Jiang X, Zhou Z, Yuan H, Zhang Z, Dong H. Sci Rep. 2014 Jul 11;4:5637.
Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD. - Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD.
Mizumura K, Cloonan SM, Nakahira K, Bhashyam AR, Cervo M, Kitada T, Glass K, Owen CA, Mahmood A, Washko GR, Hashimoto S, Ryter SW, Choi AM. J Clin Invest. 2014 Sep;124(9):3987-4003.
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Wang Y, Wang JW, Xiao X, Shan Y, Xue B, Jiang G, He Q, Chen J, Xu HG, Zhao RX, Werle KD, Cui R, Liang J, Li YL, Xu ZX. Cell Death Dis. 2013 Oct 3;4:e824.
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Sabatel C, Malvaux L, Bovy N, Deroanne C, Lambert V, Gonzalez ML, Colige A, Rakic JM, Noel A, Martial JA, Struman I. PLoS One. 2011 Feb 10;6(2):e16979.
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Gunther C, Martini E, Wittkopf N, Amann K, Weigmann B, Neumann H, Waldner MJ, Hedrick SM, Tenzer S, Neurath MF, Becker C. Nature. 2011 Sep 14;477(7364):335-9.