RIPK3

Gene Symbol RIPK3
Entrez Gene 11035
Alt Symbol RIP3
Species Human
Gene Type protein-coding
Description receptor-interacting serine-threonine kinase 3
Other Description RIP-3|RIP-like protein kinase 3|receptor interacting protein 3|receptor-interacting protein 3|receptor-interacting serine/threonine-protein kinase 3
Swissprots C4AM87 Q5J795 B4DJL9 Q5J796 Q9Y572 Q6P5Y1
Accessions EAW66021 EAW66022 Q9Y572 AF156884 AAD39005 AK075275 AK296140 BAG58881 AK296309 BAG59007 AY453693 AAS16359 AY494982 AAS75516 AY494983 AAS75517 BC041668 BC062584 AAH62584 BE676675 DB274842 NM_006871 NP_006862
Function Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. {ECO:0000269|PubMed:19498109, ECO:0000269|PubMed:19524512, ECO:0000269|PubMed:19524513, ECO:0000269|PubMed:22265413, ECO:0000269|PubMed:22265414, ECO:0000269|PubMed:22421439}.
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:22265413}. Cell membrane {ECO:0000250}. Mitochondrion {ECO:0000305}.
Tissue Specificity Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. {ECO:0000269|PubMed:15896315}.
Top Pathways Cytosolic DNA-sensing pathway, TNF signaling pathway

RIP3 (E1Z1D) Rabbit mAb - 13526 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1Z1D
Western blot analysis of extracts from various cell lines using RIP3 (E1Z1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

RIP3 Antibody - 12107 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

RIP3 (B-2) - sc-374639 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RIP3 (B-2): sc-374639. Western blot analysis of RIP3 expression in non-transfected (A) and human RIP3 transfected (B) whole cell lysates.

RIP3 (N-14) - sc-47368 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RIP3 (N-14): sc-47368. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.

RIP3 (M-42) - sc-135171 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RIP3 (M-42): sc-135171. Western blot analysis of RIP3 expression in human small intestine tissue extract.

RIP3 (Rippy-3) - sc-56228 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
RIP3 (Rippy-3): sc-56228. Western blot analysis of RIP3 expression in non-transfected (A) and human RIP3 transfected (B) 293T whole cell lysates.

Anti-RIP3 antibody - ab152130 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RIP3 antibody (ab152130) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-RIP3 antibody - ab56164 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
RIP3 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RIP3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56164.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 65kDa; RIP3.

Anti-RIP3 antibody - ab72106 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of RIP3 expression in paraffin embedded human pancreatic tissue using ab72106 at 10ug/ml.

Anti-RIP3 antibody [2B10] - ab180535 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B10
All lanes : Anti-RIP3 antibody [2B10] (ab180535) at 1/2000 dilutionLane 1 : Lysate from HEK293T cells transfected with pCMV6-ENTRY control cDNA for 48 hoursLane 2 : Lysate from HEK293T cells transfected with pCMV6-ENTRY RIP3 cDNA for 48 hoursLysates/proteins at 5 µg per lane.SecondaryAnti-mouse HRP conjugated antibodydeveloped using the ECL technique

Anti-RIP3 antibody [Rippy-3] - ab16090 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Rippy-3
All lanes : Anti-RIP3 antibody [Rippy-3] (ab16090) at 1/1000 dilutionLane 1 : HEK 293T cells(5x105) mock transfected Lane 2 : HEK 293T cells (5x105)transfected with an expression plasmid coding for human RIP3

Anti-RIP3 antibody - C-terminal - ab135070 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-RIP3 antibody - C-terminal (ab135070) at 1/500 dilution (2hrs at RT) + HeLa total cell extract

Anti-RIP3 antibody - N-terminal - ab135640 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-RIP3 antibody - N-terminal (ab135640) at 1/50 dilution + Ramos cell line lysate at 35 µg

Anti-RIPK3 / RIP3 Antibody (N-Terminus, Azide-free, HRP) - LS-C282297 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RIPK3 / RIP3 Antibody (aa50-272) - LS-C296194 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant RIPK3 / RIP3.

Anti-RIPK3 / RIP3 Antibody (aa233-518, clone 1B3) - LS-C338650 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B3
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min).

Anti-RIPK3 / RIP3 Antibody (aa61-91) - LS-C99072 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-RIPK3 / RIP3 Antibody (aa233-518, clone 2B10) - LS-C336790 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B10
IHC of paraffin-embedded Human Ovary tissue using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-RIPK3 / RIP3 Antibody - LS-C48294 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RIPK3 / RIP3 Antibody (clone Rippy-3) - LS-C53528 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Rippy-3

Anti-RIPK3 / RIP3 Antibody (aa473-486) - LS-C93312 from LifeSpan Bioscience

Prices $650.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RIPK3 / RIP3 Antibody (aa168-217) - LS-C31844 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
RIPK3 / RIP3 antibody LS-C31844 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.

Anti-RIPK3 / RIP3 Antibody (aa473-486) - LS-C189187 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RIPK3 / RIP3 Antibody (aa38-87) - LS-C80027 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-RIPK3 / RIP3 Antibody - LS-C18665 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
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