Western blot analysis of extracts from various cell lines using CHD4 (D4B7) Rabbit mAb.
Confocal immunofluorescent analysis of 293T cells using CHD4 (D4B7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 K562 cells and either 10 μl of CHD4 (D4B7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516,