CHD4

Gene Symbol CHD4
Entrez Gene 1108
Alt Symbol CHD-4, Mi-2b, Mi2-BETA
Species Human
Gene Type protein-coding
Description chromodomain helicase DNA binding protein 4
Other Description ATP-dependent helicase CHD4|Mi-2 autoantigen 218 kDa protein|chromodomain-helicase-DNA-binding protein 4
Swissprots Q8IXZ5 Q14839
Accessions EAW88777 EAW88778 EAW88779 Q14839 AA099889 AK128741 BAG54725 BC014964 AAH14964 BC025962 AAH25962 BC038596 AAH38596 BP234473 CR936680 EU446505 ABZ92034 X86691 CAA60384 XM_005253668 XP_005253725 XM_006718958 XP_006719021 XM_006718959 XP_006719022 XM_006718960 XP_006719023 XM_006718961 XP_006719024 XM_006718962 XP_006719025 NM_001273 NP_001264 NM_001297553 NP_001284482
Function Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. {ECO:0000269|PubMed:17626165, ECO:0000269|PubMed:9804427}.
Subcellular Location Nucleus {ECO:0000269|PubMed:17626165}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:17626165}. Note=Associates with centrosomes in interphase. {ECO:0000250|UniProtKB:Q6PDQ2}.
Top Pathways Viral carcinogenesis

CHD4 (D8B12) Rabbit mAb - 11912 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8B12
Western blot analysis of extracts from various cell lines using CHD4 (D8B12) Rabbit mAb.

CHD4 Antibody - 4245 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 and NCCIT cells using CHD4 Antibody.

CHD4 (D4B7) Rabbit mAb - 12011 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4B7
Western blot analysis of extracts from various cell lines using CHD4 (D4B7) Rabbit mAb.

Mi2 (B-4) - sc-55606 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mi2 (B-4): sc-55606. Western blot analysis of Mi2 expression in M1 whole cell lysate.

Mi2 (H-242) - sc-11378 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Mi2 (H-242): sc-11378. Western blot analysis of Mi2 expression in K-562 nuclear extract.

Mi2-β (F-3) - sc-365638 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mi2-β (F-3): sc-365638. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Mi2-β (C-17) - sc-8774 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Mi2-β (C-17): sc-8774. Western blot analysis of Mi2-β expression in Jurkat (A) and K-562 (B) nuclear extracts.

Mi2-β (F-7) - sc-365639 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mi2-β (F-7): sc-365639. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Mi2-β (H-7) - sc-393647 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mi2-β (H-7): sc-393647. Western blot analysis of Mi2-β expression in K-562 (A), Jurkat (B) and HeLa (C) nuclear extracts.

Mi2-β (H-8) - sc-373820 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mi2-β (H-8): sc-373820. Western blot analysis of Mi2-β expression in Jurkat (A) and K-562 (B) nuclear extracts.

Mi2-β (S-13) - sc-12541 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Mi2-β (S-13): sc-12541. Western blot analysis of Mi2-β expression in K-562 (A) and Jurkat (B) nuclear extracts.

Mi2-β (SW-88) - sc-134386 from Santa Cruz Biotechnology

Prices $295.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Mi2-β (SW-88): sc-134386. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human transitional cell carcinoma tissue showing nuclear localization.

Anti-CHD4 antibody - ab54603 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
CHD4 antibody (ab54603) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human transitional cell carcinoma.

Anti-CHD4 antibody - ab85620 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CHD4 antibody - ab110971 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HepG2 cells, using CHD4 antibody (ab110971). On the left without blocking peptide and on the right with blocking peptide.

Anti-CHD4 antibody - ab90178 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CHD4 antibody (ab90178) at 1 µg/ml + OVCAR-3 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-CHD4 antibody [3F2/4] (FITC) - ab187793 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3F2/4

Anti-CHD4 antibody [3F2/4] (HRP) - ab187792 from Abcam

Prices $372.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3F2/4

Anti-CHD4 antibody [3F2/4] - ChIP Grade - ab70469 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F2/4
ICC/IF image of ab70469 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70469, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CHD4 antibody [EPR12228] - ab171950 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR12228
Flow cytometric analysis of permeabilized Jurkat cells labeling CHD4 with ab171950 at 1/10 dilution (red) or a rabbit IgG negative control antibody (green).

Anti-CHD4 antibody [EPR12229] - ab181370 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12229
All lanes : Anti-CHD4 antibody [EPR12229] (ab181370) at 1/10000 dilutionLane 1 : NCCIT cell lysatesLane 2 : 293T cell lysatesLane 3 : HeLa cell lysatesLane 4 : Jurkat cell lysatesLysates/proteins at 10 µg per lane.

Anti-CHD4 antibody - C-terminal - ab135124 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CHD4 antibody - C-terminal (ab135124) at 1/1000 dilutionLane 1 : HeLa total cell extractLane 2 : Mouse spleen total cell extract

Anti-CHD4 antibody - ChIP Grade - ab72418 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CHD4 antibody - ChIP Grade (ab72418) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-CHD4 Antibody IHC-plus™ - LS-B204 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-CHD4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B204 concentration 20 ug/ml.

Anti-CHD4 Antibody (aa71-120) IHC-plus™ - LS-B5605 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-CHD4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5605 concentration 5 ug/ml.
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