Anti-RNF168 antibody
| Name | Anti-RNF168 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58063 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB ICC/IF ICC/IF FC IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 462-572 of Human RNF168 |
| Description | Mouse Monoclonal |
| Gene | RNF168 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-RNF168 antibody (ab58063) at 1 µg/ml (HepG2 cell lysate)
Immunohistochemical analysis formalin/PFA-fixed paraffin-enbedded Human prostate cancer tissue labelling RNF168 with ab58063 at 1.5μg/ml.
Immunofluorescence analysis of HepG2 cells labelling RNF168 with ab58063 at 10μg/ml.
![Overlay histogram showing HepG2 cells stained with ab58063 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58063, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min) /permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23828_RNF168-Primary-antibodies-ab58063-1.jpg)
Overlay histogram showing HepG2 cells stained with ab58063 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58063, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min) /permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
DNA damage to a single chromosome end delays anaphase onset. - DNA damage to a single chromosome end delays anaphase onset.
Silva BA, Stambaugh JR, Yokomori K, Shah JV, Berns MW. J Biol Chem. 2014 Aug 15;289(33):22771-84.
K63-linked ubiquitination of FANCG is required for its association with the - K63-linked ubiquitination of FANCG is required for its association with the
Zhu B, Yan K, Li L, Lin M, Zhang S, He Q, Zheng D, Yang H, Shao G. Oncogene. 2014 Aug 18.
Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. - Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
Nakada S, Tai I, Panier S, Al-Hakim A, Iemura S, Juang YC, O'Donnell L, Kumakubo A, Munro M, Sicheri F, Gingras AC, Natsume T, Suda T, Durocher D. Nature. 2010 Aug 19;466(7309):941-6.