
All lanes : Anti-RNF20 antibody (ab33500) at 1/500 dilutionLane 1 : 20 µg Human hTERT fibroblasts whole cell lysateLane 2 : 20 µg Human hTERT fibroblasts whole cell lysate transfected with RNF20 siRNA (3 days)SecondaryGoat anti-Rabbit conjugated to HRP at 1/10000 dilutionPerformed under reducing conditions.Observed band size : 120 kDa (why is the actual band size different from the predicted?)Additional bands at : 150 kDa (possible non-specific binding),170 kDa (possible non-specific binding),60 kDa (possible non-specific binding).This image is courtesy of an Abreview submitted by Dr Kirk McManus. Please refer to abreview for further experimental details.See Abreview