RNF20

Gene Symbol RNF20
Entrez Gene 56254
Alt Symbol BRE1, BRE1A, hBRE1
Species Human
Gene Type protein-coding
Description ring finger protein 20, E3 ubiquitin protein ligase
Other Description BRE1 E3 ubiquitin ligase homolog|BRE1-A|E3 ubiquitin-protein ligase BRE1A|homolog of S. cerevisiae BRE1
Swissprots Q9HA51 Q9NUR4 Q9H9Y7 Q8N3J4 Q5VTR2 Q6P527 Q96JD3 A7MCT5 Q9NX83 Q2TB34 Q69YL5 Q9NWQ3
Accessions AAG24713 EAW58955 EAW58956 EAW58957 Q5VTR2 AF265230 AAK58539 AF283791 AAG44865 AF305515 AAG21410 AK000389 BAA91134 AK000697 BAA91326 AK002051 BAA92057 AK022300 BAB14005 AK022532 BAB14081 AK314401 BAG37025 AL832910 CAH10630 AL834272 CAD38947 AY006321 AAG15824 BC016691 AAH16691 BC032618 AAH32618 BC063115 AAH63115 BC110584 AAI10585 BC110585 AAI10586 BC152309 AAI52310 DB273543 XM_011518862 XP_011517164 NM_019592 NP_062538
Function Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. {ECO:0000269|PubMed:16307923, ECO:0000269|PubMed:16337599, ECO:0000269|PubMed:19037095, ECO:0000269|PubMed:19410543}.
Subcellular Location Nucleus {ECO:0000269|PubMed:19037095, ECO:0000305|PubMed:16337599}.
Tissue Specificity Expressed in the normal brain and also in malignant gliomas (at protein level). {ECO:0000269|PubMed:19037095}.

RNF20 (D6E10) XP ® Rabbit mAb - 11974 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6E10
Western blot analysis of extracts from various cell lines using RNF20 (D6E10) XP ® Rabbit mAb.

RNF20 (D8C2) Rabbit mAb - 9425 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8C2
Western blot analysis of extracts from various cell lines using RNF20 (D8C2) Rabbit mAb.

RNF20 (C-12) - sc-107288 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RNF20 (Y-16) - sc-107290 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RNF20 antibody - ab70495 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RNF20 antibody (ab70495) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgdeveloped using the ECL technique

Anti-RNF20 antibody - ab33500 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab33500 (1/500) detecting RNF20 in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview

Anti-RNF20 antibody - ab89764 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-RNF20 antibody (ab89764) at 1 µg/ml + HeLa cell lysate at 50 µg

Anti-RNF20 antibody [EPR13562(B)] - ab181104 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13562(B)
Western blot analysis on immunoprecipitation of 293 cell lysate labeling RNF20 using ab181104 at a 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution and 5% NFDM/TBST blocking peptide.

Anti-RNF20 antibody [EPR13563(B)] - ab181032 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13563(B)
Western blot analysis on immunoprecipitation pellet from 293 cell lysate labeling RNF20 with ab181032 at 1/10 dilution.

Anti-RNF20 antibody [RNF20-85] - ab50534 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone RNF20-85
HeLa cells, fixed with 4% paraformaldehyde for 10 minutes. Permeabilized with 0.5% triton X-100 0.5% for 5 minutes. Ab50534 used at a concentration of 10 microgram/ml with 1%BSA for 1 hour.

Anti-RNF20 antibody - N-terminal - ab167086 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-RNF20 antibody - N-terminal (ab167086) at 1 µg/mlLane 2 : Anti-RNF20 antibody - N-terminal (ab167086) at 2 µg/mlLane 1 : Human liver tissue lysateLane 2 : Human liver tissue lysateLysates/proteins at 15 µg per lane.

Anti-RNF20 Antibody (aa125-175) - LS-C93314 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa1-50) - LS-C93315 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa117-146, APC) - LS-C240133 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa117-146, AP) - LS-C240132 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa117-146, Biotin) - LS-C240134 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa117-146, PE) - LS-C240140 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa117-146, Azide-free, HRP) - LS-C248029 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa1-50) - LS-C286013 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody (aa116-146) - LS-C324740 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF20 Antibody - LS-C332064 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-RNF20 Antibody (aa576-625) - LS-C80557 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.

Anti-RNF20 Antibody (aa117-146) - LS-C162655 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RNF20 Antibody western blot of 293 cell line lysates (35 ug/lane). The RNF20 antibody detected the RNF20 protein (arrow).

Anti-RNF20 Antibody (aa108-157) - LS-C102016 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate.

Anti-RNF20 Antibody (aa117-146, FITC) - LS-C240135 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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