Anti-RNF20 antibody [EPR13562(B)]

• Supplier: Abcam
• Catalog: ab181104
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13562(B)
• Applications: ICC/IF ICC/IF IP FC WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RNF20 aa 1-100
• Description: Rabbit Monoclonal
• Gene: RNF20
• Conjugate: Unconjugated

Product images

Western blot analysis on immunoprecipitation of 293 cell lysate labeling RNF20 using ab181104 at a 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution and 5% NFDM/TBST blocking peptide.

Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling RNF20 with ab181104 at 1/290 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/150 dilution.

Immonofluorescence analysis of 4% paraformaldehyde fixed MCF7 cells, labeling RNF20 using ab181104 at a 1/250 dilution (green) followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution and counterstained with Dapi (blue).

All lanes : Anti-RNF20 antibody [EPR13562(B)] (ab181104) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution