Anti-RNF4 antibody
| Name | Anti-RNF4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab166680 |
| Prices | $370.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, corresponding to internal sequence amino acids of Mouse RNF4 with a 26 kDa proprietary tag (UniProt: Q9QZS2) |
| Description | Rabbit Polyclonal |
| Gene | RNF4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab166680 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166680, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-RNF4 antibody (ab166680) at 1/1000 dilution (at RT for 2h) + Mouse brain total cell extract