RNF4

Gene Symbol RNF4
Entrez Gene 6047
Alt Symbol RES4-26, SLX5, SNURF
Species Human
Gene Type protein-coding
Description ring finger protein 4
Other Description E3 ubiquitin ligase RNF4|E3 ubiquitin-protein ligase RNF4|small nuclear RING finger protein
Swissprots D6RF58 P78317 Q49AR8 B2R6D6
Accessions EAW82522 EAW82523 EAW82524 EAW82525 EAW82526 P78317 AB000468 BAA19122 AK128038 BAG54620 AK307372 AK309509 AK311314 AK312534 BAG35433 BC031935 AAH31935 BU682428 CB993989 DA342512 DB064628 EU446651 ABZ92180 U95140 AAC52022 NM_001185009 NP_001171938 NM_001185010 NP_001171939 NM_002938 NP_002929
Function E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation. {ECO:0000269|PubMed:12885770, ECO:0000269|PubMed:18408734, ECO:0000269|PubMed:19307308, ECO:0000269|PubMed:20026589, ECO:0000269|PubMed:2021
Subcellular Location Cytoplasm. Nucleus. Nucleus, PML body. Nucleus, nucleoplasm.
Tissue Specificity Widely expressed at low levels in many tissues; highly expressed in testis. {ECO:0000269|PubMed:9479498}.

RNF4 (C-15) - sc-21351 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RNF4 antibody - ab166680 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab166680 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166680, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RNF4 Antibody (aa95-123) IHC-plus™ - LS-B10482 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-RNF4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10482 dilution 1:100.

Anti-RNF4 Antibody (aa94-123, AP) - LS-C268431 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF4 Antibody (aa94-123, APC) - LS-C268432 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF4 Antibody (aa94-123, Biotin) - LS-C268433 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF4 Antibody (aa94-123, FITC) - LS-C285076 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF4 Antibody (aa94-123, Azide-free, HRP) - LS-C285077 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF4 Antibody (aa94-123, PE) - LS-C285078 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF4 Antibody (aa94-123) - LS-C324746 from LifeSpan Bioscience

Prices $435.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

RNF4 Antibody - H00006047-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: RNF4 Antibody [H00006047-A01] - Western Blot detection against Immunogen (35.35 KDa)

RNF4 Antibody - NBP2-13243 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: RNF4 Antibody [NBP2-13243] Staining of human cell line U-2 OS shows positivity in cytoskeleton (microtubules).

Anti-RNF4 (176-190) antibody produced in rabbit - SAB1100322 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-RNF4 (176-190), <B>Cat. No. SAB1100322</B>: Immunofluorescence of HUVEC cells using Anti-RNF4 (176-190), SAB1100322 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-RNF4 antibody produced in rabbit - HPA049356 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RNF4:<B>Cat. No. HPA049356</B>: Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-RNF4 (11-25) antibody produced in rabbit - SAB1100321 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-RNF4 (11-25), <B>Cat. No. SAB1100321</B>: Immunofluorescence of HUVEC cells using Anti-RNF4 (11-25), SAB1100321 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

RNF4-Antibody-C-term - AP16278b from Abgent, a WuXi AppTec company

Prices $95.00, $265.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RNF4 Antibody (C-term)at 1:2000 dilution + human kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

RNF4 Antibody - C-terminal region (OAAB11685) - OAAB11685 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

RNF4 Antibody - abx028709 from Abbexa

Host Rabbit
Clonality Polyclonal

RNF4 (C-term) antibody - AP53693PU-N from Acris Antibodies

Prices $300.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis in T47D cell line lysates (35µg/lane) using RNF4 Antibody (C-term) Cat.-No AP53693PU-N. This demonstrates the RNF4 antibody detected the RNF4 protein (arrow).

RNF4 antibody - H00006047-A01 from Acris Antibodies

Prices $300.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Rabbit Anti-Human RNF4 (C-term) - 102-12922 from RayBiotech

Host Rabbit
RNF4 Antibody (C-term) (Cat# 102-12922) western blot analysis in LNCaP cell line lysates (35ug/lane).This demonstrates the RNF4 antibody detected the RNF4 protein (arrow).

RNF4 antibody (C-term) - STJ44818 from St John's Laboratory Ltd

Host Rabbit
Clonality Polyclonal
Clone RB35579

RNF4 antibody - 17810-1-AP from Proteintech Group

Prices $279.00
Sizes con: 48 μg/150μl
Host Rabbit
Clonality Polyclonal
IP Result of anti-RNF4 (IP:17810-1-AP, 4ug; Detection:17810-1-AP 1:300) with PC-3 cells lysate 3200ug.

RNF4 - MBS6002692 from MyBioSource

Prices $485.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

RNF4, Polyclonal Antibody - MBS248767 from MyBioSource

Prices $495.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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