Anti-RNF43 antibody
| Name | Anti-RNF43 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab84125 |
| Prices | $376.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Bovine, Cat, Dog |
| Antigen | Synthetic peptide designed within residues: DFDPLVYCSP KGDPQRVDMQ PSVTSRPRSL DSVVPTGETQ VSSHVHYHRH, corresponding to internal sequence amino acids 504 - 553 of Human RNF43 (NP_060233) Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | RNF43 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-RNF43 antibody (ab84125) at 1 µg/ml + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab84125 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.