Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue labeling RNF7 withab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse kidney lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution