RNF7

Gene Symbol RNF7
Entrez Gene 9616
Alt Symbol CKBBP1, ROC2, SAG
Species Human
Gene Type protein-coding
Description ring finger protein 7
Other Description CKII beta-binding protein 1|RING-box protein 2|rbx2|regulator of cullins 2|sensitive to apoptosis gene protein|sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2|zinc RING finger protein SAG
Swissprots A8MTB5 Q9BXN8 D3DNF8 A8K1H9 Q9Y5M7 D3DNF7 C9JYL3 Q9UBF6
Accessions EAW78991 EAW78992 EAW78993 EAW78994 EAW78995 EAW78996 Q9UBF6 AF092878 AAD25962 AF142060 AAD30147 AF164679 AAD55984 AF312226 AAK37450 AK289894 BAF82583 AK311982 BAG34921 AW150174 BC005966 AAH05966 BC008627 AAH08627 BF439211 BG715867 BG716023 BG720797 BG742338 BT007348 AAP36012 BU959624 CA415974 DB272382 DQ891759 ABM82685 DQ894943 ABM85869 NM_001201370 NP_001188299 NM_014245 NP_055060 NM_183237 NP_899060 NR_037702 NR_037703 NM_183063
Function Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents. {ECO:0000269|PubMed:10851089}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:10082581}. Nucleus {ECO:0000269|PubMed:10082581}.
Tissue Specificity Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung. {ECO:0000269|PubMed:10512750}.
Top Pathways Ubiquitin mediated proteolysis

Rbx2 (G-8) - sc-166554 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rbx2 (G-8): sc-166554. Western blot analysis of Rbx2 expression in non-transfected: sc-110760 (A) and human Rbx2 transfected: sc-110905 (B) 293 whole cell lysates.

Rbx2 (H-62) - sc-292565 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rbx2 (H-62): sc-292565. Western blot analysis of Rbx2 expression in non-transfected: sc-110760 (A) and human Rbx2 transfected: sc-110905 (B) 293 whole cell lysates.

Rbx2 (N-15) - sc-5203 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rbx2 (N-15): sc-5203. Western blot analysis of Rbx2 expression in non-transfected: sc-110760 (A) and human Rbx2 transfected: sc-110905 (B) 293 whole cell lysates.

Rbx2 (S-16) - sc-5204 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RNF7 antibody - ab34873 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF7 antibody [EPR12001] - ab181986 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12001
Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

Anti-ROC2 antibody - ab174690 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed, paraffin embedded Human Kidney tissue labeling ROC2 with ab174690 at 5 µg/ml.

Anti-RNF7 Antibody (C-Terminus) - LS-C19102 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF7 Antibody - LS-C317993 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF7 Antibody - LS-C285600 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF7 Antibody - LS-C285601 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF7 Antibody (Biotin) - LS-C317989 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF7 Antibody (FITC) - LS-C317990 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TRIP / LRRFIP1 Antibody (HRP) - LS-C317170 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF7 Antibody (HRP) - LS-C317996 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TRIP / LRRFIP1 Antibody (Biotin) - LS-C318826 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RNF7 Antibody - LS-C346345 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of MCF7 cell using RNF7 antibody. Blue: DAPI for nuclear staining.

Anti-RNF7 Antibody (aa59-71) IHC-plus™ - LS-B8759 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-RNF7 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

RNF7 Antibody - NBP1-85594 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RNF7 Antibody [NBP1-85594] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

RNF7 Antibody - NBP1-78049 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RNF7 Antibody - NBP1-46159 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: RNF7 Antibody [NBP1-46159] - Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Rabbit Anti-RNF7 Polyclonal Antibody - bs-7570R from Bioss

Prices $245.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-RNF7/CKBBP1 Polyclonal Antibody, Unconjugated (bs-7570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-RNF7 antibody produced in goat - SAB2501463 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RNF7 <B>Cat. No. SAB2501463</B>: (1 μg/mL) staining of Human Heart lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-RNF7 (21-35) antibody produced in rabbit - SAB1104221 from Sigma-Aldrich

Prices $285.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human RNF7 was separated on SDS-PAGE, blotted with Anti-RNF7 (21-35) (<B>Cat. No. SAB1104221</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + RNF7 immunizing peptide (human, 21-35)

Anti-RNF7 antibody produced in rabbit - HPA036995 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RNF7: <B>Cat. No. HPA036995</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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