All lanes : Anti-RNF86 antibody [4E5] (ab123899) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfec ted with pCMV6-ENTRY RNF86 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-RNF86 antibody [4E5] (ab123899) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
Flow cytometric analysis of HeLa cells, using ab123899 at 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).