Anti-Robo1 antibody
| Name | Anti-Robo1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7279 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF IHC-F IHC-P WB ELISA IP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide: C-VLGGYERGEDNNE conjugated to KLH, corresponding to amino acids 1632-1644 of Human Robo1 |
| Description | Rabbit Polyclonal |
| Gene | ROBO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab7279 at 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C. An Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody was used as the secondary.See Abreview
ab7279 at a 1/2000 dilution staining Robo1 in mouse muscle satellite cells by Immunohistochemistry/ Immunofluorescence, incubated for 12 hours at +4°C. PFA fixed. Blocked with 5% serum for 1 hour at 20°C. Secondary used at 1/500 monoclonal Goat anti-rabbit conjugated to Alexa Fluor 594 (red).See Abreview
ab7279 staining Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with ab7279 for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.
Anti-Robo1 antibody (ab7279) at 1/1000 dilution + Mouse brain lysate at 35 µgSecondaryGoat anti-Rabbit (H7L) at 1/10000 dilutionPerformed under reducing conditions.Observed band size : 181 kDa (why is the actual band size different from the predicted?)
Product References
Endocannabinoids modulate cortical development by configuring Slit2/Robo1 - Endocannabinoids modulate cortical development by configuring Slit2/Robo1
Alpar A, Tortoriello G, Calvigioni D, Niphakis MJ, Milenkovic I, Bakker J, Cameron GA, Hanics J, Morris CV, Fuzik J, Kovacs GG, Cravatt BF, Parnavelas JG, Andrews WD, Hurd YL, Keimpema E, Harkany T. Nat Commun. 2014 Jul 17;5:4421.
USP33 mediates Slit-Robo signaling in inhibiting colorectal cancer cell - USP33 mediates Slit-Robo signaling in inhibiting colorectal cancer cell
Huang Z, Wen P, Kong R, Cheng H, Zhang B, Quan C, Bian Z, Chen M, Zhang Z, Chen X, Du X, Liu J, Zhu L, Fushimi K, Hua D, Wu JY. Int J Cancer. 2015 Apr 15;136(8):1792-802.
Slit2 overexpression results in increased microvessel density and lesion size in - Slit2 overexpression results in increased microvessel density and lesion size in
Guo SW, Zheng Y, Lu Y, Liu X, Geng JG. Reprod Sci. 2013 Mar;20(3):285-98.
Gene expression and functional annotation of the human ciliary body epithelia. - Gene expression and functional annotation of the human ciliary body epithelia.
Janssen SF, Gorgels TG, Bossers K, Ten Brink JB, Essing AH, Nagtegaal M, van der Spek PJ, Jansonius NM, Bergen AA. PLoS One. 2012;7(9):e44973.
MiR-218 suppresses nasopharyngeal cancer progression through downregulation of - MiR-218 suppresses nasopharyngeal cancer progression through downregulation of
Alajez NM, Lenarduzzi M, Ito E, Hui AB, Shi W, Bruce J, Yue S, Huang SH, Xu W, Waldron J, O'Sullivan B, Liu FF. Cancer Res. 2011 Mar 15;71(6):2381-91.
A Slit/miR-218/Robo regulatory loop is required during heart tube formation in - A Slit/miR-218/Robo regulatory loop is required during heart tube formation in
Fish JE, Wythe JD, Xiao T, Bruneau BG, Stainier DY, Srivastava D, Woo S. Development. 2011 Apr;138(7):1409-19.
The role of SLIT-ROBO signaling in proliferative diabetic retinopathy and retinal - The role of SLIT-ROBO signaling in proliferative diabetic retinopathy and retinal
Zhou W, Yu W, Xie W, Huang L, Xu Y, Li X. Mol Vis. 2011;17:1526-36. Epub 2011 Jun 8.
The corticofugal neuron-associated genes ROBO1, SRGAP1, and CTIP2 exhibit an - The corticofugal neuron-associated genes ROBO1, SRGAP1, and CTIP2 exhibit an
Ip BK, Bayatti N, Howard NJ, Lindsay S, Clowry GJ. Cereb Cortex. 2011 Jun;21(6):1395-407.
Vascular Robo4 restricts proangiogenic VEGF signaling in breast. - Vascular Robo4 restricts proangiogenic VEGF signaling in breast.
Marlow R, Binnewies M, Sorensen LK, Monica SD, Strickland P, Forsberg EC, Li DY, Hinck L. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10520-5. doi:
Systematic analysis of a novel human renal glomerulus-enriched gene expression - Systematic analysis of a novel human renal glomerulus-enriched gene expression
Lindenmeyer MT, Eichinger F, Sen K, Anders HJ, Edenhofer I, Mattinzoli D, Kretzler M, Rastaldi MP, Cohen CD. PLoS One. 2010 Jul 12;5(7):e11545.