ROBO1

Gene Symbol ROBO1
Entrez Gene 6091
Alt Symbol DUTT1, SAX3
Species Human
Gene Type protein-coding
Description roundabout, axon guidance receptor, homolog 1 (Drosophila)
Other Description deleted in U twenty twenty|roundabout homolog 1
Swissprots Q9BUS7 B2RXI1 Q9Y6N7 Q7Z300 Q1RMC7 E9PD49 D3DU36
Accessions EAW68884 EAW68885 EAW68886 EAW68887 EAW68888 Q9Y6N7 AF040990 AAC39575 AK025535 AK095256 AK297481 BAG59902 AK299920 BAH13173 BC001969 AAH01969 BC029676 BC112336 AAI12337 BC115020 AAI15021 BC115022 AAI15023 BC157861 AAI57862 BC171855 AAI71855 BP228659 BX538319 CAD98093 DR003097 XM_006713276 XP_006713339 XM_006713277 XP_006713340 XM_011533976 XP_011532278 XM_011533977 XP_011532279 XM_011533978 XP_011532280 XM_011533979 XP_011532281 XM_011533980 XP_011532282 NM_001145845 NP_001139317 NM_002941 NP_002932 NM_133631 NP_598334
Function Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. {ECO:0000269|PubMed:10102268}.
Subcellular Location Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.
Tissue Specificity Widely expressed, with exception of kidney. {ECO:0000269|PubMed:9608531}.
Top Pathways Axon guidance

robo1 (H-200) - sc-25672 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
robo1 (H-200): sc-25672. Western blot analysis of robo1 expression in JM1 whole cell lysate.

robo1 (I-20) - sc-16612 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
robo1 (I-20): sc-16612. Western blot analysis of robo1 expression in JM1 whole cell lysate.

robo1 (N-20) - sc-16611 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Robo1 antibody - ab7279 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab7279 at 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C. An Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody was used as the secondary.See Abreview

Anti-Robo1 antibody - ab85312 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Robo1 antibody (ab85312) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Robo1 antibody - ab58297 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Robo1 antibody [MM0892-14Q9] - ab201632 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0892-14Q9

Anti-Robo1 antibody - C-terminal - ab184622 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human breast tissue, labeling Robo1 with ab184622 at 1/100 dilution.

Anti-ROBO1 Antibody (aa1275-1325) - LS-C93402 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ROBO1 Antibody (aa1632-1644) - LS-C93404 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ROBO1 Antibody (aa1601-1651) - LS-C93403 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-ROBO1 Antibody (aa491-589) - LS-C126240 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ROBO1 Antibody (aa19-560) - LS-C126241 from LifeSpan Bioscience

Prices $575.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal

Anti-ROBO1 Antibody (aa1601-1651) - LS-C288075 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ROBO1 Antibody (aa1275-1325) - LS-C288090 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ROBO1 Antibody (clone 1F8) - LS-C139227 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F8
Detection limit for recombinant GST tagged ROBO1 is approximately 0.1 ng/ml as a capture antibody.

Anti-ROBO1 Antibody - LS-C140586 from LifeSpan Bioscience

Prices $460.00
Sizes 10 blot
Host Rabbit
Clonality Polyclonal
Western blot of ROBO1 antibody (LS-C140586) on HT-29 cell lysate. Lysate used at 30 ug/lane. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP, used at 1:50k dilution.

Anti-ROBO1 Antibody - LS-C140597 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of ROBO1 antibody (LS-C140597) on human brain lysate. Lysate used at 15 ug/lane. Antibody used at 10 ug/ml. Secondary antibody, mouse anti-rabbit HRP, used at 1:50k.

Anti-ROBO1 Antibody (aa1417-1428) IHC-plus™ - LS-B12224 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Mouse Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-ROBO1 Antibody (aa994-1006) IHC-plus™ - LS-B3011 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-ROBO1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3011 concentration 2 ug/ml.

Anti-ROBO1 Antibody (aa1632-1644) IHC-plus™ - LS-B81 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B81 concentration 5 ug/ml.

ROBO1 Antibody - NB600-1254 from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: ROBO1 Antibody [NB600-1254] - Lane 1: HeLa WCL. Load: 30 ug per lane. Primary antibody: Robo-1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 181 kDa, 181 kDa for Robo-1. Other band(s): not identified.

ROBO1 Antibody (770502) - MAB71181 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 770502

ROBO1 Antibody - AF7118 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7118) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ROBO1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ROBO1 Antibody (770506) - MAB7118 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 770506
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROBO1 Monoclonal Antibody (Catalog # MAB7118) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROBO1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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