Anti-ROCK1 antibody [EP786Y]

• Supplier: Abcam
• Catalog: ab45171
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP786Y
• Applications: IHC-F WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Bovine, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ROCK1 aa 1100-1200 (C terminal)
• Description: Rabbit Monoclonal
• Gene: ROCK1
• Conjugate: Unconjugated

Product images

All lanes : Anti-ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution (purified)Lane 1 : HeLa cell lysate - treated with Calyculin ALane 2 : HeLa cell lysate - treated with CamptothecinLane 3 : HeLa cell lysateLane 4 : Jurkat cell lysateLane 5 : Ramos cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution (purified)Lane 1 : PC-12 cell lysateLane 2 : RAW264.7 cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-ROCK1 antibody [EP786Y] (ab45171) at 1/500 dilution (unpurified)Lane 1 : Untreated HeLa cell lysateLane 2 : Camptothecin treated HeLa lysate

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of the colon tissue labelling ROCK1 with purified ab45171 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Unpurified ab45171 staining ROCK1 in mouse embyro 18dpc tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS in TBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling ROCK1 with unpurified ab45171.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ROCK1 with purified ab45171 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/50) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ROCK1 using unpurified ab45171.

Overlay histogram showing HeLa cells stained with unpurified ab45171 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45171, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

ab45171 (purified) at 1/40 immunoprecipitating ROCK1 in HeLa cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Product References

Serglycin proteoglycan promotes apoptotic versus necrotic cell death in mast - Serglycin proteoglycan promotes apoptotic versus necrotic cell death in mast

Melo FR, Grujic M, Spirkoski J, Calounova G, Pejler G. J Biol Chem. 2012 May 25;287(22):18142-52.

Tumour suppressor microRNA-584 directly targets oncogene Rock-1 and decreases - Tumour suppressor microRNA-584 directly targets oncogene Rock-1 and decreases

Ueno K, Hirata H, Shahryari V, Chen Y, Zaman MS, Singh K, Tabatabai ZL, Hinoda Y, Dahiya R. Br J Cancer. 2011 Jan 18;104(2):308-15.

ROCK2 is involved in accelerated fetal lung development induced by in vivo lung - ROCK2 is involved in accelerated fetal lung development induced by in vivo lung

Cloutier M, Tremblay M, Piedboeuf B. Pediatr Pulmonol. 2010 Oct;45(10):966-76.

Potentiation of nerve growth factor-induced neurite outgrowth by the ROCK - Potentiation of nerve growth factor-induced neurite outgrowth by the ROCK

Minase T, Ishima T, Itoh K, Hashimoto K. Eur J Pharmacol. 2010 Dec 1;648(1-3):67-73.

Shh and ROCK1 modulate the dynamic epithelial morphogenesis in circumvallate - Shh and ROCK1 modulate the dynamic epithelial morphogenesis in circumvallate

Kim JY, Lee MJ, Cho KW, Lee JM, Kim YJ, Kim JY, Jung HI, Cho JY, Cho SW, Jung HS. Dev Biol. 2009 Jan 1;325(1):273-80.

Extraction of membrane cholesterol disrupts caveolae and impairs serotonergic - Extraction of membrane cholesterol disrupts caveolae and impairs serotonergic

Sommer B, Montano LM, Carbajal V, Flores-Soto E, Ortega A, Ramirez-Oseguera R, Irles C, El-Yazbi AF, Cho WJ, Daniel EE. Can J Physiol Pharmacol. 2009 Mar;87(3):180-95.