Anti-ROCK2 antibody
| Name | Anti-ROCK2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66320 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human, Chicken, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1350 to the C-terminus of Human ROCK2 |
| Description | Rabbit Polyclonal |
| Gene | ROCK2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ROCK2 antibody (ab66320) at 1 µg/mlLane 1 : Liver (Mouse) Tissue LysateLane 2 : SK N BE (Human neuroblastoma) Whole Cell LysateLane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 4 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLane 6 : Liver (Rat) Tissue LysateLane 7 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)Lane 8 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 9 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)Lane 10 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab66320 stained HeLa cells. The cells were 10% neutral buffered formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66320, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab66320 staining ROCK2 in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66320, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Rho kinase enhances contractions of rat mesenteric collecting lymphatics. - Rho kinase enhances contractions of rat mesenteric collecting lymphatics.
Kurtz KH, Souza-Smith FM, Moor AN, Breslin JW. PLoS One. 2014 Apr 7;9(4):e94082.
Activation of ErbB2 and Downstream Signalling via Rho Kinases and ERK1/2 - Activation of ErbB2 and Downstream Signalling via Rho Kinases and ERK1/2
Akhtar S, Yousif MH, Dhaunsi GS, Sarkhouh F, Chandrasekhar B, Attur S, Benter IF. PLoS One. 2013 Jun 27;8(6):e67813. Print 2013.