Anti-ROCK2 antibody (ab137697) at 1/1000 dilution + whole cell lysate prepared from H1299 cells at 30 µg
All lanes : Anti-ROCK2 antibody (ab137697) at 1/500 dilutionLane 1 : Whole cell lysate prepared from NIH-3T3 cellsLane 2 : Whole cell lysate prepared from JC cellsLysates/proteins at 30 µg per lane.
Anti-ROCK2 antibody (ab137697) at 1/1000 dilution + rat brain lysate at 50 µg
Immunofluorescence analysis of methanol-fixed A431, using ab137697 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded esophagous CA, using ab137697 at 1/100 dilution.