Anti-ROCK2 antibody (ab183636) at 1/1000 dilution + Rat2 whole cell lysate at 30 µg
All lanes : Anti-ROCK2 antibody (ab183636) at 1/1000 dilutionLane 1 : Neuro 2A whole cell lysateLane 2 : GL261 whole cell lysateLane 3 : C8D30 whole cell lysateLane 4 : NIH 3T3 whole cell lysateLane 5 : BCL-1 whole cell lysateLane 6 : Raw 264.7 whole cell lysateLane 7 : C2Cl2 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of methanol-fixed A431 cells labeling ROCK2 with ab183636 at 1/500 dilution (Green). Blue: Hoechst 33342 staining.