ab125025 staining ROCK2 in Mouse hippocamus brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 10% Goat serum + 2% Horse serum + 1% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.See Abreview
All lanes : Anti-ROCK2 antibody [EPR7141(B)] (ab125025) at 1/10000 dilutionLane 1 : Hela cell lysateLane 2 : L6 cell lysateLane 3 : A673 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab125025, at 1/50 dilution, staining ROCK2 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD