Anti-ROR alpha antibody
| Name | Anti-ROR alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab82289 |
| Prices | $378.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T216 of human ROR alpha |
| Description | Rabbit Polyclonal |
| Gene | RORA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence of HeLa cells labelling ROR alpha with ab82289. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab82289 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ROR alpha immunoreactivity is localized to the nucleus.
Anti-ROR alpha antibody (ab82289) at 1/50 dilution + A2058 cell line lysates at 35 µg