Anti-ROS antibody

• Supplier: Abcam
• Catalog: ab5512
• Prices: $390.00
• Sizes: 400 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P ELISA WB
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH, corresponding to amino acids 39-57 of Human ROS
• Description: Rabbit Polyclonal
• Gene: ROS1
• Conjugate: Unconjugated

Product images

ab5512 staining ROS in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

ICC/IF image of ab5512 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5512, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

A quantitative proteomic approach to identify significantly altered protein - A quantitative proteomic approach to identify significantly altered protein

Banville N, Burgess JK, Jaffar J, Tjin G, Richeldi L, Cerri S, Persiani E, Black JL, Oliver BG. PLoS One. 2014 Aug 18;9(8):e105365.

Aurora A regulates prometaphase progression by inhibiting the ability of RASSF1A - Aurora A regulates prometaphase progression by inhibiting the ability of RASSF1A

Song SJ, Song MS, Kim SJ, Kim SY, Kwon SH, Kim JG, Calvisi DF, Kang D, Lim DS. Cancer Res. 2009 Mar 15;69(6):2314-23.

Epigenetic regulation of c-ROS receptor tyrosine kinase expression in malignant - Epigenetic regulation of c-ROS receptor tyrosine kinase expression in malignant

Jun HJ, Woolfenden S, Coven S, Lane K, Bronson R, Housman D, Charest A. Cancer Res. 2009 Mar 15;69(6):2180-4.