Immunohistochemistry analysis of Human breast carcinoma using ab87278 at 1/250 dilution, with mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right). Detection: DAB staining
All lanes : Anti-RPA32/RPA2 (phospho S33) antibody (ab87278) at 1/200 dilutionLane 1 : Whole cell lysate prepared from human U2OS cells untreatedLane 2 : Whole cell lysate prepared from human U2OS cells treated with 20µM Cisplatin for 24 hoursLysates/proteins at 50 µg per lane.SecondaryIRDye 800CW conjugated goat polyclonal at 1/3000 dilution