Anti-RPA32/RPA2 antibody [9A1]

• Supplier: Abcam
• Catalog: ab125681
• Prices: $387.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 9A1
• Applications: WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Human, Monkey
• Antigen: Recombinant full length Human RPA32/RPA2 produced in HEK293T cells (NP_002937)
• Description: Mouse Monoclonal
• Gene: RPA2
• Conjugate: Unconjugated

Product images

All lanes : Anti-RPA32/RPA2 antibody [9A1] (ab125681) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RPA32/RPA2 cDNALysates/proteins at 5 µg per lane.

All lanes : Anti-RPA32/RPA2 antibody [9A1] (ab125681) at 1/1000 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human colon tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human liver tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human lung tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human thyroid tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human prostate tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human bladder tissue by Immunohistochemistry.

ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.

ab125681 at 1/100 dilution staining RPA32/RPA2 in COS7 cells transiently transfected with pCMV6-ENTRY RPA32/RPA2 by Immunofluorescence.

ab125681 at 2µg/500µl used for immunoprecipiatation of DDK tagged RPA32/RPA2 overexpression lysates (lane 2) (at 1/5 dilution with HEK293T lysate). Lane 1: Negative control (without ab125681). Secondary: 20µl (0.1mg) of goat anti-Mouse conjugated magnetic beads were mixed and incubated overnight.

ab125681 at 1/100 dilution staining RPA32/RPA2 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

ab125681 at 1/100 dilution staining RPA32/RPA2 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).