Anti-RPA32/RPA2 antibody [9H8]
| Name | Anti-RPA32/RPA2 antibody [9H8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2175 |
| Prices | $404.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 9H8 |
| Applications | FC WB IP IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | RPA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2175 - immunohistochemistryFormalin fixed paraffin embedded human tonsil stained with RPA using ABC and AEC chromogen.
ab2175 at 1/200 staining human skin fibroblasts by ICC/IF. The cells were treated with 2mM hydroxyurea for 16 hours, formaldehyde fixed and permeabilized with 0.5% Triton X100. They were then incubated with the primary antibody for 1.5 hours at 37°C. A Cy3 ® conjugated sheep anti-mouse antibody was used as the secondary.See Abreview
![All lanes : Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/mlLane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate Lane 2 : SK N BE (Human neuroblastoma) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24488_RPA32RPA2-Primary-antibodies-ab2175-2.jpg)
All lanes : Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/mlLane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate Lane 2 : SK N BE (Human neuroblastoma) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
![Anti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.Secondary antibody: anti-mouse Molecular Probes Alexa Fluor® 488 Conjugated at 1/200 dilution.See Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24489_RPA32RPA2-Primary-antibodies-ab2175-124.jpg)
Anti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.Secondary antibody: anti-mouse Molecular Probes Alexa Fluor® 488 Conjugated at 1/200 dilution.See Abreview
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining RPA32/RPA2 with ab2175. Staining was detected using DAB.
![Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24491_RPA32RPA2-Primary-antibodies-ab2175-15.jpg)
Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Nucleosome acidic patch promotes RNF168- and RING1B/BMI1-dependent H2AX and H2A - Nucleosome acidic patch promotes RNF168- and RING1B/BMI1-dependent H2AX and H2A
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Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and - Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and
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DNA-damage tolerance mediated by PCNA*Ub fusions in human cells is dependent on - DNA-damage tolerance mediated by PCNA*Ub fusions in human cells is dependent on
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The efficiency of homologous recombination and non-homologous end joining systems - The efficiency of homologous recombination and non-homologous end joining systems
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