Phospho-Chk1 (Ser317) (D12H3) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12302
• Prices: $119.00, $318.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D12H3
• Applications: WB IP ICC/IF
• Species Reactivities: Human, Mouse, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser317 of human Chk1 protein.
• Description: Rabbit Monoclonal
• Gene: CHEK1

Product images

Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated (-) or UV-treated (100 mJ, 1 hr recovery; +), using Phospho-Chk1 (Ser317) (D12H3) XP ® Rabbit mAb. The blot on the right was treated with calf intestinal phosphatase (CIP) before western blot.

Immunoprecipitation of phospho-Chk1 (Ser317) from 293 cell extracts treated with UV (100 mJ, 1 hr recovery) using Phospho-Chk1 (Ser317) (D12H3) XP ® Rabbit mAb (lane 2) or Rabbit (D1AG) mAb IgG XP ® Isotype Control #3900 (lane 3). Lane 1 is 10% input.

Confocal immunofluorescent analysis of HeLa cells, untreated (left), UV-treated (center), or UV and λ phosphatase-treated (right), using Phospho-Chk1 (Ser317) (D12H3) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).