CHEK1

Gene Symbol CHEK1
Entrez Gene 1111
Alt Symbol CHK1
Species Human
Gene Type protein-coding
Description checkpoint kinase 1
Other Description CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase|checkpoint kinase-1|serine/threonine-protein kinase Chk1
Swissprots B5BTY6 B4DSK3 O14757 H2BI51 B4DDD0 A8K934 F5H7S4
Accessions AAM78553 AAQ76038 BAA84577 EAW67644 O14757 AA224205 AB451222 BAG70036 AB451345 BAG70159 AF016582 AAC51736 AF032874 AAB88852 AI750792 AK292549 BAF85238 AK293143 BAG56691 AK299783 BAG61665 AK300082 BAG61885 AW149126 BC004202 AAH04202 BC017575 AAH17575 BG191840 BG216281 BG218896 BG468734 BM455102 BP203333 BP207812 BX114781 BX419129 BX506561 CA441277 CA868104 DA567616 DQ892907 ABM83833 DQ896142 ABM87141 JF289264 AEB71796 XM_011542560 XP_011540862 XM_011542561 XP_011540863 XM_011542562 XP_011540864 XM_011542563 XP_011540865 NM_001114121 NP_001107593 NM_001114122 NP_001107594 NM_001244846 NP_001231775 NM_001274 NP_001265 NR_045204 NR_045205
Function Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser- 124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of C
Subcellular Location Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.
Tissue Specificity Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. {ECO:0000269|PubMed:9278511, ECO:0000269|PubMed:9382850}.
Top Pathways Cell cycle, p53 signaling pathway, HTLV-I infection, Viral carcinogenesis

Phospho-Chk1 (Ser317) (D12H3) XP ® Rabbit mAb (PE Conjugate) - 13959 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D12H3
Flow cytometric analysis of SK-MEL-28 cells, untreated (blue) or neocarzinostatin-treated (green), using Phospho-Chk1 (Ser317) (D12H3) XP® Rabbit mAb (PE Conjugate).

Phospho-Chk1 (Ser345) (133D3) Rabbit mAb (PE Conjugate) - 12268 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 133D3
Flow cytometric analysis of HeLa cells, untreated (blue) and UV-treated (green), using Phospho-Chk1 (Ser345) (133D3) Rabbit mAb (PE Conjugate).

Phospho-Chk1 (Ser317) (D12H3) XP ® Rabbit mAb - 12302 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D12H3
Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated (-) or UV-treated (100 mJ, 1 hr recovery; +), using Phospho-Chk1 (Ser317) (D12H3) XP ® Rabbit mAb. The blot on the right was treated with calf intestinal phosphatase (CIP) before western blot.

Phospho-Chk1 (Ser345) Antibody - 2341 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells treated with UV or MMS for the indicated times using Phospho-Chk1 (Ser345) Antibody.

Phospho-Chk1 (Ser317) Antibody - 2344 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and COS cells treated with HU or UV as indicated, using Phospho-Chk1 (Ser317) Antibody.

Phospho-Chk1 (Ser280) Antibody - 2347 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT29 cells, untreated or UV-treated, using Phospho-Chk1 (Ser280) Antibody (upper) or Chk1 Antibody #2345 (lower).

Phospho-Chk1 (Ser296) Antibody - 2349 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Hela and Cos cells, untreated or treated with 100 mJ/cm 2 UV light with 1 hour recovery, using Phospho-Chk1 (Ser296) Antibody.

Phospho-Chk1 (Ser317) (D7H2) Rabbit mAb - 8191 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7H2
Western blot analysis of extracts from HeLa or 293 cells, untreated (-) or treated with UV (100 mJ) (+), using Phospho-Chk1 (Ser317) (D7H2) Rabbit mAb (upper) or Chk1 (2G1D5) Mouse mAb #2360 (lower).

Phospho-Chk1 (Ser345) (133D3) Rabbit mAb - 2348 from Cell Signaling Technology

Prices $109.00, $307.00, $719.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 133D3
Western blot analysis of extracts from HeLa, COS, NIH/3T3 and C6 cells, untreated or UV-treated, using Phospho-Chk1 (Ser345) (133D30) Rabbit mAb.

Chk1 (2G1D5) Mouse mAb - 2360 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2G1D5
Western blot analysis of extracts from various cell lines using Chk1 (2G1D5) Mouse mAb.

Chk1 Antibody - 2345 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-Chk1 (Ser296) Antibody - 2346 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Chk1 (D-7) - sc-377231 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk1 (D-7): sc-377231. Western blot analysis of Chk1 expression in HeLa (A) and K-562 (B) whole cell lysates.

Chk1 (DCS-310) - sc-56291 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk1 (DCS-310): sc-56291. Western blot analysis of Chk1 expression in HeLa (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.

Chk1 (FL-476) - sc-7898 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Chk1 (FL-476): sc-7898. Western blot analysis of Chk1 expression in HeLa (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.

Chk1 (C-16) - sc-7234 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Chk1 (C-16): sc-7234. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts, Leydig cells and peritubular myoid cells.

Chk1 (G-4) - sc-8408 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk1 siRNA (h): sc-29269. Western blot analysis of Chk1 expression in non-transfected control (A) and Chk1 siRNA transfected (B) HeLa cells. Blot probed with Chk1 (G-4): sc-8408. GAPDH (V-18): sc-20357 used as specificity and loading control.

Chk1 (H-215) - sc-292584 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Chk1 (2G11D5) - sc-56288 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk1 (2G11D5): sc-56288. Western blot analysis of Chk1 expression in HeLa (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.

p-Chk1 (Ser 345) - sc-21866 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Chk1 (Ser 345) - sc-17922 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Chk1 (Ser 280) - sc-135636 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Chk1 (Ser 280): sc-135636. Western blot analysis of phosphorylated Chk1 expression in untreated (A) and UV-treated (B) HL-60 cell extracts.

Anti-Chk1 (phospho S280) antibody - ab59988 from Abcam

Prices $380.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chk1 (phospho S280) antibody (ab59988) at 1/100 dilutionLane 1 : A2058 cell lysateLane 2 : mouse brain lysateLane 3 : Ramos cell lysateLane 4 : Jurkat cell lysateLane 5 : HL60 cell lysate Lane 6 : Hela cell lysate

Anti-Chk1 (phospho S280) antibody - ab47560 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chk1 (phospho S280) antibody (ab47560) at 1/500 dilutionLane 1 : HT29 cells, untreated Lane 2 : HT29 cells treated with UV

Anti-Chk1 (phospho S280) antibody - ab192430 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chk1 (phospho S280) antibody (ab192430) at 1/500 dilutionLane 1 : UV treated HT29 whole cell lysates treated with the antigen-specific peptideLane 2 : UV treated HT29 whole cell lysatesLysates/proteins at 12.5 µg per lane.
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