Anti-RRM1 antibody
| Name | Anti-RRM1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab81085 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF IP IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide derived from the C-terminus of human RRM1 |
| Description | Rabbit Polyclonal |
| Gene | RRM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab81085, at 1/100 dilution, staining RRM1 in Human Placenta by Immunohistochemistry using formalin-fixed paraffin-embedded tissue.
Anti-RRM1 antibody (ab81085) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![RRM1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RRM1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81085.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..Band: Band: 90kDa: RRM1.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25361_RRM1-Primary-antibodies-ab81085-5.jpg)
RRM1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RRM1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81085.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..Band: Band: 90kDa: RRM1.
Immunohistochemical analysis of Human pancreatic ductal adenocarcinoma tissue, staining RRM1 with ab81085 at 1/50 dilution.
Product References
Variation in drug sensitivity of malignant mesothelioma cell lines with - Variation in drug sensitivity of malignant mesothelioma cell lines with
Szulkin A, Nilsonne G, Mundt F, Wasik AM, Souri P, Hjerpe A, Dobra K. PLoS One. 2013 Jun 20;8(6):e65903.
Is there a role for the quantification of RRM1 and ERCC1 expression in pancreatic - Is there a role for the quantification of RRM1 and ERCC1 expression in pancreatic
Valsecchi ME, Holdbrook T, Leiby BE, Pequignot E, Littman SJ, Yeo CJ, Brody JR, Witkiewicz AK. BMC Cancer. 2012 Mar 22;12:104.
Ribonucleotide reductase inhibition restores platinum-sensitivity in - Ribonucleotide reductase inhibition restores platinum-sensitivity in
Kunos C, Radivoyevitch T, Abdul-Karim FW, Fanning J, Abulafia O, Bonebrake AJ, Usha L. J Transl Med. 2012 Apr 27;10:79.
Hypoxia-related protein expression and its clinicopathologic implication in - Hypoxia-related protein expression and its clinicopathologic implication in
Koo JS, Kim H. Tumour Biol. 2011 Oct;32(5):893-904.