Anti-RRM2 antibody
| Name | Anti-RRM2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57653 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 1-111 of Human RRM2 |
| Description | Mouse Monoclonal |
| Gene | RRM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
RRM2 antibody (ab57653) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.
ICC/IF image of ab57653 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57653, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25391_RRM2-Primary-antibodies-ab57653-2.jpg)
Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
The expression of ribonucleotide reductase M2 in the carcinogenesis of uterine - The expression of ribonucleotide reductase M2 in the carcinogenesis of uterine
Su YF, Wu TF, Ko JL, Tsai HT, Tee YT, Chien MH, Chou CH, Lin WL, Low HY, Chou MY, Yang SF, Wang PH. PLoS One. 2014 Mar 17;9(3):e91644.
Inhibition of hepatocellular carcinoma growth using immunoliposomes for - Inhibition of hepatocellular carcinoma growth using immunoliposomes for
Gao J, Chen H, Yu Y, Song J, Song H, Su X, Li W, Tong X, Qian W, Wang H, Dai J, Guo Y. Biomaterials. 2013 Dec;34(38):10084-98.
Ribonucleotide reductase inhibition restores platinum-sensitivity in - Ribonucleotide reductase inhibition restores platinum-sensitivity in
Kunos C, Radivoyevitch T, Abdul-Karim FW, Fanning J, Abulafia O, Bonebrake AJ, Usha L. J Transl Med. 2012 Apr 27;10:79.