All lanes : Anti-RSK4 antibody (ab42100) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.