Anti-RSPO4 antibody - C-terminal (ab175244) at 1/100 dilution + Jurkat cell lysate at 15 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling RSPO4 with ab175244 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells labeling RSPO4 with ab175244 at 1/10 dilution (bottom histogram)compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.