Anti-Rubicon antibody
| Name | Anti-Rubicon antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92388 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-F |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape |
| Antigen | Synthetic peptide, from a region within amino acids 922-972 of Human Rubicon (NP_055502 |
| Description | Rabbit Polyclonal |
| Gene | KIAA0226 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Rubicon antibody (ab92388) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
Sample: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). Antibodies: ab92388 used for WB 1 µg/ml (lane 1) and used for IP at 3 µg/mg lysate. Lane 2: control IgG. Detection: chemiluminescence with exposure time of 30 seconds.
ICC/IF image of ab92388 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92388, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of Human renal cancer tissue, staining Rubicon with ab92388. Tissue was fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.
Immunohistochemical analysis of Human renal tumour cells, staining Rubicon with ab92388. Cells were fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.
Product References
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Bruntz RC, Taylor HE, Lindsley CW, Brown HA. J Biol Chem. 2014 Jan 10;289(2):600-16.
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Banerjee P, Basu A, Wegiel B, Otterbein LE, Mizumura K, Gasser M, Waaga-Gasser AM, Choi AM, Pal S. J Biol Chem. 2012 Sep 14;287(38):32113-23.