KIAA0226

Gene Symbol KIAA0226
Entrez Gene 9711
Alt Symbol RUBICON, SCAR15
Species Human
Gene Type protein-coding
Description KIAA0226
Other Description RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein|RUN domain protein as Beclin 1-interacting and cysteine-rich containing|baron|beclin-1 associated RUN domain containing protein|run domain Beclin-1 interacting and cystein-rich containing protein|run domain Beclin-1 interacting and cysteine-rich containing protein|rundataxin
Swissprots Q96CK5 Q92622
Accessions EAW92238 EAW92239 Q92622 AI796534 AK295625 BAG58502 BC014173 AAH14173 BC033615 AAH33615 BC160011 AAI60011 BU501990 BX647598 CAI46107 CN288121 D86979 BAA13215 DA007321 DB036516 DB075342 HY003277 XM_005269374 XP_005269431 XM_006713827 XP_006713890 XM_006713828 XP_006713891 XM_006713829 XP_006713892 XM_006713830 XP_006713893 XM_006713831 XP_006713894 NM_001145642 NP_001139114 NM_014687 NP_055502
Function Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity (By similarity). {ECO:0000250}.
Subcellular Location Late endosome {ECO:0000269|PubMed:19270696}. Lysosome {ECO:0000269|PubMed:19270696}. Early endosome {ECO:0000269|PubMed:19270696}. Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus.

Rubicon (D8B2) Rabbit mAb - 7151 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8B2
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human Rubicon (hRubicon, +), using Rubicon (D8B2) Rabbit mAb. Rubicon construct was kindly provided by Dr. Qing Zhong, University of California, Berkeley, CA.

Rubicon (D9F7) Rabbit mAb - 8465 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9F7
Western blot analysis of extracts from various cell lines using Rubicon (D9F7) Rabbit mAb.

KIAA0226 (N-17) - sc-99955 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
KIAA0226 (N-17): sc-99955. Western blot analysis of KIAA0226 expression in non-transfected: sc-117752 (A) and mouse KIAA0226 transfected: sc-121210 (B) 293T whole cell lysates.

Anti-Rubicon antibody - ab92388 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rubicon antibody (ab92388) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-Rubicon antibody - ab156052 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab156052 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab156052 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h.ab50533 (Mouse monoclonal [Rab7-117] to RAB7) was also incubated along with ab156052 to demonstrate antigen co-localization. Signal detected using Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed (ab150120) (pseudo-colored red)  DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Rubicon antibody - ab187016 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Rubicon antibody - ab108109 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Rubicon antibody (ab108109) at 1 µg/ml + Human placenta lysate at 10 µg

Anti-Rubicon antibody - N-terminal - ab173930 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Rubicon antibody - N-terminal (ab173930) at 1/50 dilution + MCF7 cell lysate at 35 µg

Anti-KIAA0226 / RUBICON Antibody (aa922-972) - LS-C289309 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0226 / RUBICON Antibody (aa249-278) - LS-C329254 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0226 / RUBICON Antibody (aa311-360) - LS-C116905 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
KIAA0226 antibody LS-C116905 Western blot of Placenta lysate.

Anti-KIAA0226 / RUBICON Antibody (aa241-271) - LS-C156742 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lymph with BARON Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-KIAA0226 / RUBICON Antibody (N-Terminus) - LS-C336205 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 ug/ml.

Rubicon Antibody - NBP1-30461 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: Rubicon Antibody [NBP1-30461] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-30461 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Rubicon was also immunoprecipitated by another rabbit anti-Rubicon antibody, which recognizes an upstream epitope.

Anti-KIAA0226 antibody produced in rabbit - HPA054497 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-KIAA0226: <B>Cat. No. HPA054497</B>: Immunohistochemical staining of Human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Anti-KIAA0226 antibody produced in rabbit - SAB2107356 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-KIAA0226: <B>Cat. No. SAB2107356</B>: Western blot analysis of KIAA0226 with KIAA0226 (KIAA0226) antibody against the middle region of KIAA0226 (50 μg) antibody at 1.0 μg/mL.

Anti-KIAA0226 antibody produced in rabbit - HPA050908 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-KIAA0226: <B>Cat. No. HPA050908</B>: Immunohistochemical staining of Human kidney shows moderate cytoplasmic and membranous positivity in renal tubules.

KIAA0226 antibody - middle region (ARP58950_P050) - ARP58950_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

BARON-Antibody-N-term - AP1831a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of BARON antibody (N-term) (Cat.# AP1831a) in MCF-7 cell line lysates (35ug/lane). BARON (arrow) was detected using the purified Pab.

BARON Antibody - N-terminal region (OAAB13850) - OAAB13850 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
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