Anti-RUNX1 / AML1 (phospho S303) antibody
| Name | Anti-RUNX1 / AML1 (phospho S303) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55308 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ELISA WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic phosphopeptide derived from human RUNX1/ AML1 around the phosphorylation site of serine 303 (P-I-SP-P-G) |
| Description | Rabbit Polyclonal |
| Gene | RUNX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-RUNX1 / AML1 (phospho S303) antibody (ab55308) at 1/500 dilutionLane 1 : extracts from Jurkat cells.Lane 2 : extracts from Jurkat cells, treated with the immunising phosphopeptide.Lysates/proteins at 5 µg per lane.SecondaryGoat Anti-Rabbit IgG (H+L)
Ab55308 staining human colon. Staining is localized to the nucleus.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.