RUNX1

Gene Symbol RUNX1
Entrez Gene 861
Alt Symbol AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB
Species Human
Gene Type protein-coding
Description runt-related transcription factor 1
Other Description AML1-EVI-1 fusion protein|CBF-alpha-2|PEA2-alpha B|PEBP2-alpha B|SL3-3 enhancer factor 1 alpha B subunit|SL3/AKV core-binding factor alpha B subunit|acute myeloid leukemia 1 protein|core-binding factor, runt domain, alpha subunit 2|oncogene AML-1|polyomavirus enhancer-binding protein 2 alpha B subunit
Swissprots O60473 Q16347 O76089 Q16285 Q13757 Q01196 A8MV94 Q15341 Q15343 Q13755 B2RMS4 Q13756 Q16286 Q16346 O76047 Q13759 O60472 D3DSG1 Q13081 Q13758 Q92479 Q16284
Accessions AAC05246 AAC05247 AGY36885 CAA13070 EAX09769 EAX09770 Q01196 AA878154 AK226159 AK310587 AK310646 AL581043 AV761975 BC050363 BC110828 AAI10829 BC136380 AAI36381 BC136381 AAI36382 BC144053 AAI44054 BM149149 BX475024 CA503244 CD638998 D10570 BAA01426 D43967 BAA07902 D43968 BAA07903 D43969 BAA07904 D89788 BAA14020 D89789 BAA14021 D89790 BAA14022 KF041488 KF041489 L34598 AAA51720 S60998 S76345 AAB33729 S76346 AAB33730 S76350 AAB33731 U19601 AAB51691 X79549 CAA56092 X90976 CAA62464 X90977 X90979 CAA62466 X90980 CAA62467 X90981 CAA62468 XM_005261068 XP_005261125 XM_005261069 XP_005261126 XM_011529766 XP_011528068 XM_011529767 XP_011528069 XM_011529768 XP_011528070 XM_011529769 XP_011528071 XM_011529770 XP_011528072 XR_937576 NM_001001890 NP_001001890 NM_001122607 NP_001116079 NM_001754 NP_001745
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). {ECO:0000269|PubMed:10207087, ECO:0000269|PubMed:11965546, ECO:0000269|PubMed:14970218, ECO:0000269|PubMed:17377532, ECO:0000269|PubMed:17431401}.
Subcellular Location Nucleus.
Tissue Specificity Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
Top Pathways Chronic myeloid leukemia, Acute myeloid leukemia, Transcriptional misregulation in cancer

Phospho-AML1 (Ser249) Antibody - 4327 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of TPA treated HEL cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (upper), or AML1 Antibody #4334 (lower).

AML1 (D33G6) XP ® Rabbit mAb - 4336 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D33G6
Western blot analysis of extracts from various cell lines using AML1 (D33G6) XP ® Rabbit mAb.

AML1 Antibody - 4334 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and Kasumi-1 cells, using AML1 Antibody.

AML1 Antibody (Mouse Preferred) - 8229 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using AML1 Antibody (Mouse Preferred).

AML1 (D4A6) Rabbit mAb (Mouse Preferred) - 8529 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4A6
Western blot analysis of extracts from EL4 cells using AML1 (D4A6) Rabbit mAb (Mouse Preferred).

RUNX1 (A-2) - sc-365644 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RUNX1 (A-2): sc-365644. Western blot analysis of RUNX1 expression in Jurkat nuclear extract.

RUNX1 (C-19) - sc-8564 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RUNX1 (C-19): sc-8564. Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear staining of respiratory epithelial cells.

RUNX1 (H-65) - sc-28679 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

RUNX1 (N-20) - sc-8563 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RUNX1 (DW71) - sc-101146 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
RUNX1 (DW71): sc-101146. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta tissue showing nuclear localization.

p-RUNX1 (12.Ser 249) - sc-293146 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of RUNX1 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) Jurkat nuclear extracts. Antibodies tested include p-RUNX1 (12.Ser 249): sc-293146 (A,B) and RUNX1 (A-2): sc-365644 (C,D).

Anti-RUNX1 / AML1 (phospho S303) antibody - ab55308 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RUNX1 / AML1 (phospho S303) antibody (ab55308) at 1/500 dilutionLane 1 : extracts from Jurkat cells.Lane 2 : extracts from Jurkat cells, treated with the immunising phosphopeptide.Lysates/proteins at 5 µg per lane.SecondaryGoat Anti-Rabbit IgG (H+L)

Anti-RUNX1 / AML1 antibody - ab35962 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
developed using the ECL technique

Anti-RUNX1 / AML1 antibody - ab68939 from Abcam

Host Rabbit
Clonality Polyclonal
Immunofluorescence on mouse cerebellum (4% PFA fixed, frozen section) at 1/500 dilution using rabbit antibody to human, rat, mouse Runt-related transcription factor 1 (Runx1, AML1, CBFA2): whole serum. DAPI counterstain appearing blue.

Anti-RUNX1 / AML1 antibody - ab79808 from Abcam

Host Sheep
Clonality Polyclonal

Anti-RUNX1 / AML1 antibody - ab55363 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RUNX1 / AML1 antibody (ab55363) at 1/500 dilutionLane 1 : extracts from Jurkat cells with no immunizing peptideLane 2 : extracts from Jurkat cells with immunizing peptide

Anti-RUNX1 / AML1 antibody - ab55998 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RUNX1 / AML1 antibody [5A1] - ab110035 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5A1
Anti-RUNX1 / AML1 antibody [5A1] (ab110035) at 1/500 dilution + Jurkat cell lysate.

Anti-RUNX1 / AML1 antibody [RUNXA3B6] - ab53491 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone RUNXA3B6
Anti-RUNX1 / AML1 antibody [RUNXA3B6] (ab53491) + Immunising peptide

Anti-RUNX1 / AML1 antibody - ChIP Grade - ab23980 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1/1000 dilutionLane 1 : Mouse BladderLane 2 : Mouse LungLane 3 : Mouse LiverLane 4 : Mouse ThymusSecondaryDonkey polyclonal to anti-Rabbit IgG, HRP-Linked F(ab')2 Fragment at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Runx1 (phospho S276) antibody - ab182659 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Runx1 (phospho S276) antibody (ab182659) at 1/500 dilutionLane 1 : JK cell extract with antigen-specific peptideLane 2 : JK cell extract

Anti-Runx1 (phospho S435) antibody - ab193645 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Runx1 (phospho S435) antibody (ab193645) at 1/500 dilutionLane 1 : Extracts from 293 cells with antigen-specific peptideLane 2 : Extracts from 293 cellsLane 3 : Extracts from HeLa cellsLane 4 : Extracts from HepG2 cells

Anti-Runx1 (phospho S435) antibody - ab138377 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Runx1 (phospho S435) antibody (ab138377) at 1/500 dilution + HepG2 cell lysate - cells treated with PMA (125ng/ml, 30 min) at 30 µg

Anti-Runx1 antibody - ab196795 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Runx1 antibody (ab196795) at 1/500 dilutionLane 1 : HepG2 cell extract, treated with PMA (125 ng/mL, 30 mins)Lane 2 : HepG2 cell extract, treated with PMA (125 ng/mL, 30 mins) with synthesized peptide

Anti-Runx1 antibody [2B5] - ab130194 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2B5
Anti-Runx1 antibody [2B5] (ab130194) at 1/500 dilution + Jurkat cell lysate
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