![Overlay histogram showing K562 cells stained with ab128084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128084, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25844_ab128084-3-ab128084FC.jpg)
Overlay histogram showing K562 cells stained with ab128084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128084, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-RUNX1T1 / ETO antibody [1H1] (ab128084) at 1/2000 dilutionLane 1 : lysate from HEK293T cells transfected with the pCMV6-ENTRY control vector for 48 hrsLane 2 : lysate from HEK293T cells transfected with the pCMV6-ENTRY RUNX1T1/ETO vector for 48 hrsLysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25845_RUNX1T1-ETO-Primary-antibodies-ab128084-1.jpg)
All lanes : Anti-RUNX1T1 / ETO antibody [1H1] (ab128084) at 1/2000 dilutionLane 1 : lysate from HEK293T cells transfected with the pCMV6-ENTRY control vector for 48 hrsLane 2 : lysate from HEK293T cells transfected with the pCMV6-ENTRY RUNX1T1/ETO vector for 48 hrsLysates/proteins at 5 µg per lane.
ab128084, at 1/100 dilution, staning RUNX1T1/ETO in COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1/ETO expression vector.