Anti-RUNX3 antibody [R3-5G4]
| Name | Anti-RUNX3 antibody [R3-5G4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40278 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | R3-5G4 |
| Applications | FC IP WB EMSA IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant fragment derived from residues 191 - 300 of Human RUNX3 |
| Description | Mouse Monoclonal |
| Gene | RUNX3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-RUNX3 antibody [R3-5G4] (ab40278) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Rabbit Anti-Mouse IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25896_ab40278_1.jpg)
All lanes : Anti-RUNX3 antibody [R3-5G4] (ab40278) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Rabbit Anti-Mouse IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
IHC image of RUNX3 staining in human lymphoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40278, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
![Overlay histogram showing HEK293 cells stained with ab40278 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40278, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25898_RUNX3-Primary-antibodies-ab40278-5.jpg)
Overlay histogram showing HEK293 cells stained with ab40278 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40278, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
RUNX family members are covalently modified and regulated by PIAS1-mediated - RUNX family members are covalently modified and regulated by PIAS1-mediated
Kim JH, Jang JW, Lee YS, Lee JW, Chi XZ, Li YH, Kim MK, Kim DM, Choi BS, Kim J, Kim HM, van Wijnen A, Park I, Bae SC. Oncogenesis. 2014 Apr 28;3:e101.
RUNX3 interacts with MYCN and facilitates protein degradation in neuroblastoma. - RUNX3 interacts with MYCN and facilitates protein degradation in neuroblastoma.
Yu F, Gao W, Yokochi T, Suenaga Y, Ando K, Ohira M, Nakamura Y, Nakagawara A. Oncogene. 2014 May 15;33(20):2601-9.
Runt-related transcription factor 3 reverses epithelial-mesenchymal transition in - Runt-related transcription factor 3 reverses epithelial-mesenchymal transition in
Tanaka S, Shiraha H, Nakanishi Y, Nishina S, Matsubara M, Horiguchi S, Takaoka N, Iwamuro M, Kataoka J, Kuwaki K, Hagihara H, Toshimori J, Ohnishi H, Takaki A, Nakamura S, Nouso K, Yagi T, Yamamoto K. Int J Cancer. 2012 Dec 1;131(11):2537-46.
RUNX3 maintains the mesenchymal phenotype after termination of the Notch signal. - RUNX3 maintains the mesenchymal phenotype after termination of the Notch signal.
Fu Y, Chang AC, Fournier M, Chang L, Niessen K, Karsan A. J Biol Chem. 2011 Apr 1;286(13):11803-13.
Src kinase phosphorylates RUNX3 at tyrosine residues and localizes the protein in - Src kinase phosphorylates RUNX3 at tyrosine residues and localizes the protein in
Goh YM, Cinghu S, Hong ET, Lee YS, Kim JH, Jang JW, Li YH, Chi XZ, Lee KS, Wee H, Ito Y, Oh BC, Bae SC. J Biol Chem. 2010 Mar 26;285(13):10122-9.
The role of EZH2 and DNA methylation in the silencing of the tumour suppressor - The role of EZH2 and DNA methylation in the silencing of the tumour suppressor
Kodach LL, Jacobs RJ, Heijmans J, van Noesel CJ, Langers AM, Verspaget HW, Hommes DW, Offerhaus GJ, van den Brink GR, Hardwick JC. Carcinogenesis. 2010 Sep;31(9):1567-75.
Frequent silencing of RUNX3 in esophageal squamous cell carcinomas is associated - Frequent silencing of RUNX3 in esophageal squamous cell carcinomas is associated
Sakakura C, Miyagawa K, Fukuda KI, Nakashima S, Yoshikawa T, Kin S, Nakase Y, Ida H, Yazumi S, Yamagishi H, Okanoue T, Chiba T, Ito K, Hagiwara A, Ito Y. Oncogene. 2007 Aug 30;26(40):5927-38. Epub 2007 Mar 26.
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing - The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing
Yano T, Ito K, Fukamachi H, Chi XZ, Wee HJ, Inoue K, Ida H, Bouillet P, Strasser A, Bae SC, Ito Y. Mol Cell Biol. 2006 Jun;26(12):4474-88.
RUNX3 protein is overexpressed in human basal cell carcinomas. - RUNX3 protein is overexpressed in human basal cell carcinomas.
Salto-Tellez M, Peh BK, Ito K, Tan SH, Chong PY, Han HC, Tada K, Ong WY, Soong R, Voon DC, Ito Y. Oncogene. 2006 Dec 7;25(58):7646-9. Epub 2006 Jun 12.