Anti-RUNX3 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab11905
• Prices: $384.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ChIP WB EMSA ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant full length protein (Human)
• Description: Rabbit Polyclonal
• Gene: RUNX3
• Conjugate: Unconjugated

Product images

Detection of AML2 by EMSA analysis. Nushift polyclonal antibody against AML2 factor creates a supershift in EMSA (lane 4).

ICC/IF image of ab11905 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11905 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Role of runt-related transcription factor 3 (RUNX3) in transcription regulation - Role of runt-related transcription factor 3 (RUNX3) in transcription regulation

Lai CB, Mager DL. J Biol Chem. 2012 Mar 2;287(10):7324-34.

Epigenetic basis for aberrant upregulation of autoantigen genes in humans with - Epigenetic basis for aberrant upregulation of autoantigen genes in humans with

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Runt-related transcription factor 3 is associated with ulcerative colitis and - Runt-related transcription factor 3 is associated with ulcerative colitis and

Weersma RK, Zhou L, Nolte IM, van der Steege G, van Dullemen HM, Oosterom E, Bok L, Peppelenbosch MP, Faber KN, Kleibeuker JH, Dijkstra G. Inflamm Bowel Dis. 2008 Dec;14(12):1615-22.

Novel roles for MLH3 deficiency and TLE6-like amplification in DNA mismatch - Novel roles for MLH3 deficiency and TLE6-like amplification in DNA mismatch

Chen PC, Kuraguchi M, Velasquez J, Wang Y, Yang K, Edwards R, Gillen D, Edelmann W, Kucherlapati R, Lipkin SM. PLoS Genet. 2008 Jun 13;4(6):e1000092.

Transcriptional repression of the RUNX3/AML2 gene by the t(8;21) and inv(16) - Transcriptional repression of the RUNX3/AML2 gene by the t(8;21) and inv(16)

Cheng CK, Li L, Cheng SH, Lau KM, Chan NP, Wong RS, Shing MM, Li CK, Ng MH. Blood. 2008 Oct 15;112(8):3391-402.