Anti-S100 antibody [4B3]
| Name | Anti-S100 antibody [4B3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab14849 |
| Prices | $400.00 |
| Sizes | 250 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 4B3 |
| Applications | WB IHC-P IHC-F ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Full length native protein (purified) (Human brain) |
| Description | Mouse Monoclonal |
| Gene | S100A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab14849 staining S100 in Human melanoma cultured cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked for 30 minutes at 21°C. Samples were incubated with primary antibody (at concentrations listed above) for 15 hours at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview
ab14849 at 1/3000 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody. A biotinylated goat anti-mouse IgG was used as the secondary.See Abreview
ab14849 staining S100 in the Human Melanoma cell line 1205Lu xenograph tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.1% Triton X100 and blocked with a commercial block solution for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris EDTA at pH 9. The sample was incubated with primary antibody (1/300 in blocking solution) at 4°C for 18 hours. A Biotin-conjugated goat polyclonal to mouse IgG (1/50) was used as secondary antibody.See Abreview
Product References
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C-type natriuretic peptide specifically acts on the pylorus and large intestine - C-type natriuretic peptide specifically acts on the pylorus and large intestine
Sogawa C, Wakizaka H, Aung W, Jin ZH, Tsuji AB, Furukawa T, Kunieda T, Saga T. Am J Pathol. 2013 Jan;182(1):172-9.
Gastrointestinal tract disorder in natriuretic peptide receptor B gene mutant - Gastrointestinal tract disorder in natriuretic peptide receptor B gene mutant
Sogawa C, Abe A, Tsuji T, Koizumi M, Saga T, Kunieda T. Am J Pathol. 2010 Aug;177(2):822-8.