S100A1

Gene Symbol S100A1
Entrez Gene 6271
Alt Symbol S100, S100-alpha, S100A
Species Human
Gene Type protein-coding
Description S100 calcium binding protein A1
Other Description S-100 protein alpha chain|S-100 protein subunit alpha|S100 alpha|S100 calcium-binding protein A1|S100 protein, alpha polypeptide|protein S100-A1
Swissprots B2R5D9 Q5T7Y3 P23297
Accessions CAY39334 EAW53301 EAW53302 P23297 AB451316 BAG70130 AB451446 BAG70260 AK126904 AK312152 BAG35086 BC014392 AAH14392 BC035791 BC072006 BC094843 BT006938 AAP35584 X58079 CAA41107 NM_006271 NP_006262
Function Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. {ECO:0000269|PubMed:22399290}.
Subcellular Location Cytoplasm.
Tissue Specificity Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

S100A1 Antibody - 5066 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using S100A1 Antibody.

S-100 (S1-61) - sc-53438 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
S-100 (S1-61): sc-53438. Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic and membrane staining of myocytes.

S-100 (1.F.2) - sc-71994 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

S-100 α chain (C-20)-R - sc-7849-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
S-100 α chain (C-20)-R: sc-7849-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.

S-100 α chain (4c4.9) - sc-58837 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
S-100 α chain (4c4.9): sc-58837. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human melanoma tissue showing cytoplasmic localization.

S-100 α chain (3B10) - sc-52204 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
S-100 α chain (3B10): sc-52204. Western blot analysis of S-100 α chain expression in non-transfected: sc-117752 (A) and mouse S-100 α chain transfected: sc-123335 (B) 293T whole cell lysates.

S-100 α chain (SH-A1) - sc-58840 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µl ascites
Host Mouse
Clonality Monoclonal
S-100 α chain (SH-A1): sc-58840. Western blot analysis of S-100 α chain expression in non-transfected: sc-117752 (A) and mouse S-100 α chain transfected: sc-123335 (B) 293T whole cell lysates.

S-100 α/β chain (B32.1) - sc-58839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

S-100 α/β chain (5E783) - sc-71990 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

S-100 α/β chain (4B3) - sc-52205 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
S-100 α/β chain (4B3): sc-52205. Western blot analysis of human recombinant S-100 β.

S-100 α/β chain (1.B.657) - sc-71991 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

Anti-S100 alpha antibody - ab189260 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-S100 alpha antibody (ab189260) at 1/500 dilutionLane 1 : A549 cell extractLane 2 : A549 cell extract with immunizing peptide

Anti-S100 alpha antibody - ab53163 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Paraffin embedded human brain tissue labelled with ab53163 at 1/50 - 1/100 dilution and treated -/+ peptide

Anti-S100 alpha antibody [2C8B8] - ab52271 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C8B8
All lanes : Anti-S100 alpha antibody [2C8B8] (ab52271)Lane 1 : Molecular weight markersLane 2 : Truncated S100A recombinant protein

Anti-S100 alpha antibody [EPR5250] - ab133519 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5250
Overlay histogram showing SHSY-5Y cells stained with ab133519 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133519, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-S100 alpha antibody [EPR5251] - ab109384 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5251
All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilutionLane 1 : Rat kidney lysateLane 2 : C2C12 lysateLane 3 : Mouse kidney lysateLane 4 : Human brain lysateLane 5 : HT-1080 lysateLane 6 : Mouse heart lysateLysates/proteins at 10 µg per lane.

Anti-S100 antibody - ab48625 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-S100 antibody - ab15520 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab15520 staining S100 at 1/100 dilution in human melanoma by Immunohistochemistry (formalin/paraffin).

Anti-S100 antibody [15E2E2] - ab86728 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 15E2E2
Overlay histogram showing SHSY-5Y cells stained with ab86728 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86728, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-S100 antibody [3B10] - ab8334 from Abcam

Prices $372.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone 3B10
Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD, Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD,

Anti-S100 antibody [4B3] - ab14849 from Abcam

Prices $400.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone 4B3
ab14849 staining S100 in Human melanoma cultured cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked for 30 minutes at 21°C. Samples were incubated with primary antibody (at concentrations listed above) for 15 hours at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview

Anti-S100 antibody [6G1] - ab85137 from Abcam

Prices $378.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 6G1
ab85137 staining S100 in the Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. ab96879, Goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.See Abreview

Anti-S100 antibody [6G1] (Biotin) - ab185170 from Abcam

Prices $372.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 6G1

Anti-S100 antibody [6G1] (HRP) - ab10203 from Abcam

Prices $376.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 6G1
S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.

Anti-S100 antibody [8B10] - ab8330 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8B10
S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.
Search more